کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8338032 | 1540986 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Exploratory study of the association of steroid profiles in stimulated ovarian follicular fluid with outcomes of IVF treatment
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کلمات کلیدی
17OHP4DHEAEstrFDRROCSFFPregnanesdehydroepiandrosteroneiAsAUC - AUCLC-MS/MS - LC-MS / MSImmunoassays - آزمایشات ایمنیandrogens - آندروژنEstradiol - استرادیولSteroids - استروئیدEstrone - استرونEstrogens - استروژنestriol - استریولallopregnanolone - الوپرگنانولونandrostenedione - اندرسوندیونtestosterone - تستوسترونlive birth - تولد زندهpregnenolone - حاملنولونMass spectrometry - طیف سنجی جرمیIn vitro fertilization - لقاح در خارج از بدن یا آیویافIVF - لقاح مصنوعی Follicular fluid - مایع فولیکولارarea under curve - منطقه تحت منحنیfalse discovery rate - میزان کشف کاذبProgesterone - پروژسترونProgestins - پروژستینGluc - گلوکزGlucocorticoids - گلوکوکورتیکوئیدهاreceiver operating characteristic - گیرنده عامل عامل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Steroid concentrations in stimulated follicular fluid (sFF) samples have been linked to the quality of oocytes used in IVF treatments. Most of the published studies focused on evaluating the association of the IVF outcomes with only a few of the steroids, measured by immunoassays (IA). We performed a treatment outcome, prospective cohort study using stimulated FF sampled from 14 infertile women undergoing IVF treatment; single oocyte was used per IVF cycle. Fourteen endogenous steroids were analyzed in 22 ovarian follicle aspirations, which corresponded to the embryos used in the IVF. Ten oocytes were associated with live birth (LB) and 12 with no pregnancy (NP). Steroids were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods. Differences in distribution of concentrations in association with the pregnancy outcome (LB or NP), and receiver operating characteristic (ROC) curves analysis were performed for the entire cohort and for within-women data. The predominant androgen and estrogen in stimulated sFF were androstenedione (A4) and estradiol (E2), respectively. Lower concentrations of pregnenolone (Pr), lower ratios of A4/ dehydroepiandrosterone (DHEA), testosterone (Te)/DHEA, and greater ratios of E2/Te, and estrone/A4 were observed in sFF samples associated with LB. Among the oocytes associated with NP, in four out of 12 samples total concentration of androgens was above the distribution of the concentrations in the oocytes corresponding to the LB group. Observations of the study indicated increased consumption of precursors and increased biosynthesis of estrogens in the follicles associated with LB. Our data suggest that potentially steroid profiles in sFF obtained during oocyte retrieval may serve as biomarkers for selection of the best embryo to transfer after IVF.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 162, September 2016, Pages 126-133
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 162, September 2016, Pages 126-133
نویسندگان
Mark M. Kushnir, Tord Naessén, Kjell Wanggren, Julius Hreinsson, Alan L. Rockwood, A.Wayne Meikle, Jonas Bergquist,