کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8338994 | 1541012 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CCAAT/enhancer-binding protein β is involved in the breed-dependent transcriptional regulation of 3β-hydroxysteroid dehydrogenase/Î5-Î4-isomerase in adrenal gland of preweaning piglets
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کلمات کلیدی
FXR3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomeraseGATA-6DAX-1STAT5BSTAT5Apeptidylprolyl isomerase AEHLPPIASF-1norepinephrine transporter3β-HSDCOX-2AGPGREc/ebpβ - c / ebpβfarnesoid X receptor - Farnesoid X گیرندهα1-Acid glycoprotein - α1-اسید گلیکوپروتئینchromatin immunoprecipitation - ایمن سازی کروماتینLarge White - بزرگ سفیدNET - خالصPig - خوکStar - ستارهcyclooxygenase 2 - سیکلوکوکسیژناز 2Steroidogenic factor 1 - عامل استروئیدوژنیک 1androgen response element - عنصر پاسخ آندروژنGlucocorticoid response element - عنصر پاسخ گلوکوکورتیکوئیدAdrenal gland - غدد فوق کلیوی، غدههای آدرنالARE - هستندCCAAT/enhancer binding protein β - پروتئین اتصال دهنده تقویت کننده CCAAT / βSteroidogenic acute regulatory protein - پروتئین حاکم استروئیدوژنیک حادCHiP - چیپAndrogen Receptor - گیرنده آندروژنیglucocorticoid receptor - گیرنده گلوکوکورتیکوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The enzyme 3β-hydroxysteroid dehydrogenase/Î5-Î4-isomerase (3β-HSD) catalyzes the biosynthesis of all steroid hormones. The molecular mechanisms regulating porcine adrenal 3β-HSD expression in different breeds are still poorly understood. In this study, we aimed to compare the expression of 3β-HSD between preweaning purebred Large White (LW) and Erhualian (EHL) piglets and to explore the potential factors regulating 3β-HSD transcription. EHL had significantly higher serum levels of cortisol (P < 0.01) and testosterone (P < 0.01), which were associated with significantly higher expression of 3β-HSD mRNA (P < 0.01) and protein (P < 0.05) in the adrenal gland, compared with LW piglets. The 5â² flanking region of the porcine 3β-HSD gene showed significant sequence variations between breeds, and the sequence of EHL demonstrated an elevated promoter activity (P < 0.05) in luciferase reporter gene assay. Higher adrenal expression of 3β-HSD in EHL was accompanied with higher CCAAT/enhancer binding protein β (C/EBPβ) expression (P < 0.05), enriched histone H3 acetylation (P < 0.05) and C/EBPβ binding to 3β-HSD promoter (P < 0.05). In addition, higher androgen receptor (AR) (P = 0.06) and lower glucocorticoid receptor (GR) (P < 0.05) were detected in EHL. Co-immunoprecipitation analysis revealed interactions of C/EBPβ with both AR and GR. These results indicate that the C/EBPβ binding to 3β-HSD promoter is responsible, at least in part, for the breed-dependent 3β-HSD expression in adrenal gland of piglets. The sequence variations of 3β-HSD promoter and the interactions of AR and/or GR with C/EBPβ may also participate in the regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 138, November 2013, Pages 273-280
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 138, November 2013, Pages 273-280
نویسندگان
Xian Li, Runsheng Li, Yimin Jia, Zhiyuan Sun, Xiaojing Yang, Qinwei Sun, Ruqian Zhao,