Estradiol-activated estrogen receptor α does not regulate mature microRNAs in T47D breast cancer cells

► 24 h E2-ERα is adequate to detect maximal transcriptional activation in T47D cells. ► microRNAs are involved in gene regulation. ► No mature miRNAs are significantly regulated by 24 h E2-ERα in T47D cells. ► Variations in miRNA regulations are as a result of biological differences.