کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8340302 | 1541225 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Robust generation and expansion of skeletal muscle progenitors and myocytes from human pluripotent stem cells
ترجمه فارسی عنوان
تولید و گسترش مستمر پروتکل های عضله اسکلتی و میوسیت ها از سلول های بنیادی پلورپوفتون انسان
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EGFPax7PSCsCHIR99021BrachyuryMyogenesisEBsFGF2RPEIPSCsDOIhESCsSMPSFACSBSA - BSAMRFs - MRF هاbovine serum albumin - آلبومین سرم گاوepithelial to mesenchymal transition - اپیتلیال به انتقال مزانشیمالGoI - بروEMT - تکنسین فوریتهای پزشکیembryoid bodies - جنین های جنینیhuman embryonic stem cells - سلول های بنیادی جنینی انسانInduced pluripotent stem cells - سلول های بنیادی پرتوان القاییPluripotent stem cells - سلول های بنیادی پلوروپتوژنEmbryonic stem cells - سلولهای بنیادی جنینیepidermal growth factor - عامل رشد اپیدرمیfibroblast growth factor 2 - عامل رشد فیبروبلاست 2Skeletal muscle - عضله اسکلتیMyogenic regulatory factors - عوامل کنترل کننده Myogenicfluorescence activated cell sorting - فلورسانس سلول فعال فعال سلولgene of interest - ژن مورد علاقهTissue culture - کشت بافت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Human pluripotent stem cells provide a developmental model to study early embryonic and tissue development, tease apart human disease processes, perform drug screens to identify potential molecular effectors of in situ regeneration, and provide a source for cell and tissue based transplantation. Highly efficient differentiation protocols have been established for many cell types and tissues; however, until very recently robust differentiation into skeletal muscle cells had not been possible unless driven by transgenic expression of master regulators of myogenesis. Nevertheless, several breakthrough protocols have been published in the past two years that efficiently generate cells of the skeletal muscle lineage from pluripotent stem cells. Here, we present an updated version of our recently described 50-day protocol in detail, whereby chemically defined media are used to drive and support muscle lineage development from initial CHIR99021-induced mesoderm through to PAX7-expressing skeletal muscle progenitors and mature skeletal myocytes. Furthermore, we report an optional method to passage and expand differentiating skeletal muscle progenitors approximately 3-fold every 2Â weeks using Collagenase IV and continued FGF2 supplementation. Both protocols have been optimized using a variety of human pluripotent stem cell lines including patient-derived induced pluripotent stem cells. Taken together, our differentiation and expansion protocols provide sufficient quantities of skeletal muscle progenitors and myocytes that could be used for a variety of studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 101, 15 May 2016, Pages 73-84
Journal: Methods - Volume 101, 15 May 2016, Pages 73-84
نویسندگان
Michael Shelton, Avetik Kocharyan, Jun Liu, Ilona S. Skerjanc, William L. Stanford,