کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8340775 | 1541254 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chromatin immunoprecipitation for human monocyte derived macrophages
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کلمات کلیدی
MCSFCCL-2PBSTNFαMDMSDSENCODEIDTPICHUVECCMLIFN-γPMSFchromatin immunoprecipitation - ایمن سازی کروماتینinterferon-gamma - اینترفرون گاماinterleukin - اینترلوکینLysis buffer - بافر لیزImmunoprecipitation - تخریب ایمنیHistone modifications - تغییرات هیستونtumor necrosis factor-alpha - تومور نکروز عامل آلفاbase pairs - جفت پایهsodium dodecyl sulfate - سدیم دودسیل سولفاتHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانmacrophage colony stimulating factor - فاکتور تحریک کننده کلون ماکروفاژPhosphate buffered saline - فسفات بافر شورPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدchronic myelogenous leukemia - لوسمی مزمن میلوئیدیchemokine ligand 2 - لیگاند شیمیایی 2Macrophage - ماکروفاژ monocyte-derived macrophages - ماکروفاژهای حاصل از مونوسیتMonocyte - مونوسیتCHiP - چیپprotease inhibitor cocktail - کوکتل مهار کننده پروتئازguanine-cytosine - گوانین-سیتوزین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The importance of Chromatin Immunoprecipitation (ChIP) technology has grown exponentially along with an increased interest in epigenetic regulation. The correlation of transcription factors with histone marks is now well established as the center of epigenetic studies; therefore, precise knowledge about histone marks is critical to unravel their molecular function and to understand their role in biological systems. This knowledge constantly accumulates and is provided openly in the expanding hubs of information such as the USCS Genome Browser. Nevertheless, as we gain more knowledge, we realize that the DNA-protein interactions are not driven by a “one size fits all” rule. Also, the diversity of interactions between DNA, histones, and transcriptional regulators is much bigger than previously considered. Besides a detailed protocol of sample preparation for the ChIP assay from primary human monocyte-derived macrophages (MDM) [an acceptable in vitro model for primary, human macrophage cells], we show that differences between various types of cells exist. Furthermore, we can postulate that such variations exist between transformed macrophage-like cell lines and primary macrophages obtained from healthy volunteers. We found that the most efficient fixation time for MDM is 10Â min. Finally, to perform multiple analytical assays, we showed that even with thorough methodology, the yield of material obtained from primary cells is the major challenge.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 70, Issues 2â3, December 2014, Pages 89-96
Journal: Methods - Volume 70, Issues 2â3, December 2014, Pages 89-96
نویسندگان
Jessica Wooden, Pawel Ciborowski,