کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8340872 | 1541266 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of human umbilical cord vein CD146+ perivascular cell origined three-dimensional vascular construct
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کلمات کلیدی
vWFl-AAPNIPAAmVCSPen-StrepDMEM-LGWST-1PGMSMCsEGMMACSAPCHUVECCTGFECMMSCsFBSFACSFITCα-SMAECs - EC هاallophycocyanin - آلوفوکسیانینAlpha-smooth muscle actin - آکتا عضله آلفا صافElastin - الاستینImmunofluorescent - ایمونوفلورسنتTem - این استumbilical cord - بند نافRoom temperature - دمای اتاقPCs - رایانه های شخصیfetal bovine serum - سرم جنین گاوHuman umbilical cord vein endothelial cells - سلول های اندوتلیال ورید بند ناف انسانMesenchymal stem cells - سلول های بنیادی مزانشیمیSmooth muscle cells - سلول های عضله صافEndothelial cells - سلولهای اندوتلیالPerivascular cells - سلولهای پریواسکولارthree dimensional - سه بعدیVon Willebrand factor - عامل فون ویلبراندVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Connective tissue growth factor - فاکتور رشد بافت همبندphycoerythrin - فایکوئیریدینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتFibrin - فیبرینExtracellular matrix - ماتریکس خارج سلولیendothelial growth medium - محیط رشد اندوتلیالSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیmagnetic activated cell sorting - مرتب سازی سلول های فعال مغناطیسیVascular tissue engineering - مهندسی بافت عروقیCell sheet engineering - مهندسی ورق سلولScanning electron microscopy - میکروسکوپ الکترونی روبشیTransmission electron microscopy - میکروسکوپ الکترونی عبوریPenicillin-streptomycin - پنیسیلین- استرپتومایسینCollagen type I - کلاژن نوع I
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Small-diameter vascular grafts are needed for the treatment of coronary artery diseases in the case of limited accessibility of the autologous vessels. Synthetic scaffolds have many disadvantages so in recent years vascular constructs (VCs) made from cellularized natural scaffolds was seen to be very promising but number of studies comprising this area is very limited. In our study, our aim is to generate fully natural triple-layered VC that constitutes all the layers of blood vessel with vascular cells. CD146+ perivascular cells (PCs) were isolated from human umbilical cord vein (HUCV) and differentiated into smooth muscle cells (SMCs) and fibroblasts. They were then combined with collagen type I/elastin/dermatan sulfate and collagen type I/fibrin to form tunica media and tunica adventitia respectively. HUCV endothelial cells (ECs) were seeded on the construct by cell sheet engineering method after fibronectin and heparin coating. Characterization of the VC was performed by immunolabeling, histochemical staining and electron microscopy (SEM and TEM). Differentiated cells were identified by means of immunofluorescent (IF) labeling. SEM and TEM analysis of VCs revealed the presence of three histologic tunicae. Collagen and elastic fibers were observed within the ECM by histochemical staining. The vascular endothelial growth factor receptor expressing ECs in tunica intima; α-SMA expressing SMCs in tunica media and; the tenascin expressing fibroblasts in tunica adventitia were detected by IF labeling. In conclusion, by combining natural scaffolds and vascular cells differentiated from CD146+ PCs, VCs can be generated layer by layer. This study will provide a preliminary blood vessel model for generation of fully natural small-diameter vascular grafts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 118, July 2018, Pages 101-112
Journal: Microvascular Research - Volume 118, July 2018, Pages 101-112
نویسندگان
Beyza Gökçinar-Yagci, Nilgün Yersal, Petek Korkusuz, Betül Ãelebi-Saltik,