کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8340922 | 1541267 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hyperglycemia induced early growth response-1 regulates vascular dysfunction in human retinal endothelial cells
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کلمات کلیدی
EGR-1HRECsNGFI-ANaBPEDFNCDVEGF-ARAGEEBsPKCDAPICBBICAM-14′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولCoomassie Brilliant Blue - Coomassie درخشان آبیDiabetes - بیماری قندdiabetic retinopathy - رتینوپاتی دیابتیEndothelial cells - سلولهای اندوتلیالHuman retinal endothelial cells - سلولهای اندوتلیال شبکیه انسانAge - سنRetina - شبکیهTissue factor - عامل بافتvascular endothelial growth factor-A - فاکتور رشد اندوتلیال عروق Apigment epithelial derived factor - فاکتور مشتق شده از اپیتلیوم رنگدانهAdvanced glycation end products - محصولات نهایی پیشرفته گلیساسیونintercellular adhesion molecule-1 - مولکول چسبندگی بین سلولی -1Hyperglycemia - هایپرگلایسمیearly growth response-1 - پاسخ سریع رشد 1Protein kinase-C - پروتئین کیناز Creceptor for AGE - گیرنده برای AGE
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Early growth response-1 (Egr-1) protein upregulation is reported in diabetes and vascular disorders. This study aims at deciphering its role in hyperglycemia induced changes of retinal endothelium. Human retinal endothelial cells (hRECs) were exposed to hyperglycemia (25Â mM) and normoglycemia (5.5Â mM). Gene silencing was done using siRNA against Egr-1. Transcript and protein level analysis of Egr-1 and gene targets were done using qPCR and immunoblotting respectively in hRECs, diabetic and nondiabetic human retina and immunofluorescence for localization in retinal sections. Hyperglycemia induced Egr-1 and vascular endothelial growth factor-A (VEGF-A) but not pigment epithelium derived factor (PEDF) in hRECs. Expression of Egr-1 repressor NGFI-A binding protein-2 (NAB-2) was unaltered. Egr-1 downstream gene targets, tissue factor (TF) and intercellular adhesion molecule-1 (ICAM-1) expression were increased in hRECs which was reduced by Egr-1 silencing in hyperglycemia. Diabetic retina, showed an increase in Egr-1, VEGF-A and gene target TF, ICAM-1 but not NAB-2 and PEDF similar to the changes seen in hyperglycemic hRECs. Hyperglycemic induction of Egr-1 and absence of NAB-2 repression in retinal endothelium, up-regulates downstream genes involved in pro-thrombotic and pro-inflammatory pathways linking Egr-1 in diabetes mediated vascular aberration of retina.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 117, May 2018, Pages 37-43
Journal: Microvascular Research - Volume 117, May 2018, Pages 37-43
نویسندگان
Gayathree Karthikkeyan, Ragavachetty Nagaraj Nareshkumar, Seeneevasan Aberami, Konerirajapuram Natarajan Sulochana, Srinivasan Vedantham, Karunakaran Coral,