کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8342896 | 1541543 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The ATP-stimulated translocation promoter (ASTP) activity of glycerol kinase plays central role in adipogenesis
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کلمات کلیدی
GA3PASTPPPPT3PDHAPGyKG6PDHVDACAdipogenesis - آدیپوژنزAcetyl CoA - استیل کولاAcoA - بلوطMetabolic flux analysis - تجزیه و تحلیل شار متابولیکdihydroxyacetone phosphate - دی هیدروکسی استون فسفاتpentose phosphate pathway - مسیر پنتوز فسفاتOxidative pentose phosphate pathway - مسیر پوسیدگی فسفات اکسیداتیوknock out - ناک اوتwild type - نوع وحشیMFA - وزارت امور خارجهMoonlighting proteins - پروتئین Moonlightingvoltage-dependent anion channel - کانال آنیون وابسته به ولتاژGlycerol kinase deficiency - کمبود گلیسرول کینازglucose-6-phosphate dehydrogenase - گلوکز 6-فسفات دهیدروژنازglyceraldehyde-3-phosphate - گلیسرالیدید-3-فسفاتGlycerol kinase - گلیسرول کیناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glycerol kinase (GK) is a multifunctional enzyme located at the interface of carbohydrate and fat metabolism. It contributes to both central carbon metabolism and adipogenesis; specifically, through its role as the ATP-stimulated translocation promoter (ASTP). GK overexpression leads to increased ASTP activity and increased fat storage in H4IIE cells. We performed metabolic flux analysis in human GK-overexpressing H4IIE cells and found that overexpressing cells had significantly altered fluxes through central carbon and lipid metabolism including increased flux through the pentose phosphate pathway and increased production of lipids. We also observed an equal contribution of glycerol to carbohydrate metabolism in all cell lines, suggesting that GK's alternate functions rather than its enzymatic function are important for these processes. To further elucidate the contributions of the enzymatic (phosphorylation) and alternative (ASTP) functions of GK in adipogenesis, we performed experiments on mammalian GK and E. coli GK. We determined that the ASTP function of GK (which is absent in E. coli GK) plays a greater role than the enzymatic activity in these processes. These studies further emphasize GK's diverse functionality and provides fundamental insights into the multiple protein functions of glycerol kinase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Genetics and Metabolism - Volume 124, Issue 4, August 2018, Pages 254-265
Journal: Molecular Genetics and Metabolism - Volume 124, Issue 4, August 2018, Pages 254-265
نویسندگان
Lilly S. Parr, Ganesh Sriram, Ramin Nazarian, Lola Rahib, Katrina M. Dipple,