کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357101 | 1542037 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of stable QTLs for seed oil content by combined linkage and association mapping in Brassica napus
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کلمات کلیدی
QTLMCMCBrassica napusCAPSRFLPAFLPRAPDPICCIMMAFSSRRandom amplified polymorphic DNA - DNA پلیمورفیک تقویت تصادفیMarkov chain Monte Carlo - زنجیره مارکف مونت کارلوLinkage disequilibrium - عدم تعادل پیوستگیquantitative trait locus - علامت کمی صفتGenome-wide association study - مطالعه مرتبط با ژنومGWAS - مطالعهٔ همخوانی سراسر ژنومcomposite interval mapping - نقشه برداری کامپوزیتAmplified fragment length polymorphism - پلی مورفیسم طول قطعه تقویت شدهrestriction fragment length polymorphism - پلی مورفیسم طول قطعه قطعهSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیLinkage mapping - پیوند نقشه برداریSNP - چندریختی تک-نوکلئوتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of stable QTLs for seed oil content by combined linkage and association mapping in Brassica napus Identification of stable QTLs for seed oil content by combined linkage and association mapping in Brassica napus](/preview/png/8357101.png)
چکیده انگلیسی
Seed oil content is an important agricultural trait in rapeseed breeding. Although numerous quantitative trait locus (QTL) have been identified, most of them cannot be applied in practical breeding mainly due to environmental instability or large confidence intervals. The purpose of this study was to identify and validate high quality and more stable QTLs by combining linkage mapping and genome-wide association study (GWAS). For linkage mapping, we constructed two F2 populations from crosses of high-oil content (â¼50%) lines 6F313 and 61616 with a low-oil content (â¼40%) line 51070. Two high density linkage maps spanned 1987Â cM (1659 bins) and 1856Â cM (1746 bins), respectively. For GWAS, we developed more than 34,000 high-quality SNP markers based on 227 accessions. Finally, 40 QTLs and 29 associations were established by linkage and association mapping in different environments. After merging the results, 32 consensus QTLs were obtained and 7 of them were identified by both mapping methods. Seven overlapping QTLs covered an average confidence interval of 183Â kb and explained the phenotypic variation of 10.23 to 24.45%. We further developed allele-specific PCR primers to identify each of the seven QTLs. These stable QTLs should be useful in gene cloning and practical breeding application.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 252, November 2016, Pages 388-399
Journal: Plant Science - Volume 252, November 2016, Pages 388-399
نویسندگان
Fengming Sun, Jing Liu, Wei Hua, Xingchao Sun, Xinfa Wang, Hanzhong Wang,