کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357583 | 1542051 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phytochrome-interacting factors PIF4 and PIF5 negatively regulate anthocyanin biosynthesis under red light in Arabidopsis seedlings
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کلمات کلیدی
FRCChIP-qPCREGL3phytochrome interacting factorTTG1Pap1GL3COP1PHYBPIFsWLCMBWLDOXCHICHSDFRqRT-PCRF3HF3′H - F3'Hflavonoid 3′-hydroxylase - flavonoid 3'-hydroxylaseHY5 - hY5Arabidopsis - آرابیدوپسیس یا رشادیElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزAnthocyanin - آنتوسیانینleucoanthocyanidin dioxygenase - دیئکسیژناز لوسآنتوسیانیدینquantitative reverse transcription PCR - رونویسی معکوس PCR کمیchalcone synthase - سنتاز چالکونEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکflavanone 3-hydroxylase - فلاونون 3-هیدروکسیلازphytochrome B - فیتوکروم BRed light - چراغ قرمزchalcone isomerase - کلومون ایزومراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Light is an important environmental factor inducing anthocyanin accumulation in plants. Phytochrome-interacting factors (PIFs) have been shown to be a family of bHLH transcription factors involved in light signaling in Arabidopsis. Red light effectively increased anthocyanin accumulation in wild-type Col-0, whereas the effects were enhanced in pif4 and pif5 mutants but impaired in overexpression lines PIF4OX and PIF5OX, indicating that PIF4 and PIF5 are both negative regulators for red light-induced anthocyanin accumulation. Consistently, transcript levels of several genes involved in anthocyanin biosynthesis and regulatory pathway, including CHS, F3â²H, DFR, LDOX, PAP1 and TT8, were significantly enhanced in mutants pif4 and pif5 but decreased in PIF4OX and PIF5OX compared to in Col-0, indicating that PIF4 and PIF5 are transcriptional repressor of these gene. Transient expression assays revealed that PIF4 and PIF5 could repress red light-induced promoter activities of F3â²H and DFR in Arabidopsis protoplasts. Furthermore, chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) test and electrophoretic mobility shift assay (EMSA) showed that PIF5 could directly bind to G-box motifs present in the promoter of DFR. Taken together, these results suggest that PIF4 and PIF5 negatively regulate red light-induced anthocyanin accumulation through transcriptional repression of the anthocyanin biosynthetic genes in Arabidopsis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 238, September 2015, Pages 64-72
Journal: Plant Science - Volume 238, September 2015, Pages 64-72
نویسندگان
Zhongjuan Liu, Yongqiang Zhang, Jianfeng Wang, Ping Li, Chengzhou Zhao, Yadi Chen, Yurong Bi,