کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357894 | 1542056 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
New insights into the wheat chromosome 4D structure and virtual gene order, revealed by survey pyrosequencing
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کلمات کلیدی
LMPISBPMDABrachypodium distachyonSyntenyNGSSSRdN/dS - DN / DSEST - استmultiple displacement amplification - تقویت جابجایی چندگانهNext generation sequencing - توالی نسل بعدیExpressed Sequence Tag - تکرار اظهار نظرgene annotation - حاشیه نویسی ژنGene content - محتوای ژنSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتیدCdS - کادمیم سولفید، سولفید کادمیمTriticum aestivum - گندم نان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Survey sequencing of the bread wheat (Triticum aestivum L.) genome (AABBDD) has been approached through different strategies delivering important information. However, the current wheat sequence knowledge is not complete. The aim of our study is to provide different and complementary set of data for chromosome 4D. A survey sequence was obtained by pyrosequencing of flow-sorted 4DS (7.2Ã) and 4DL (4.1Ã) arms. Single ends (SE) and long mate pairs (LMP) reads were assembled into contigs (223Â Mb) and scaffolds (65Â Mb) that were aligned to Aegilops tauschii draft genome (DD), anchoring 34Â Mb to chromosome 4. Scaffolds annotation rendered 822 gene models. A virtual gene order comprising 1973 wheat orthologous gene loci and 381 wheat gene models was built. This order was largely consistent with the scaffold order determined based on a published high density map from the Ae. tauschii chromosome 4, using bin-mapped 4D ESTs as a common reference. The virtual order showed a higher collinearity with homeologous 4B compared to 4A. Additionally, a virtual map was constructed and â¼5700 genes (â¼2200 on 4DS and â¼3500 on 4DL) predicted. The sequence and virtual order obtained here using the 454 platform were compared with the Illumina one used by the IWGSC, giving complementary information.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 233, April 2015, Pages 200-212
Journal: Plant Science - Volume 233, April 2015, Pages 200-212
نویسندگان
Marcelo Helguera, Máximo Rivarola, Bernardo Clavijo, Mihaela M. Martis, Leonardo S. Vanzetti, Sergio González, Ingrid Garbus, Phillippe Leroy, Hana Å imková, Miroslav Valárik, Mario Caccamo, Jaroslav Doležel, Klaus F.X. Mayer, Catherine Feuillet,