کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357962 | 1542058 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit
ترجمه فارسی عنوان
اجتناب از نور، تجمع اسید آسکوربیک در پوست مرکبات را کاهش می دهد
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کلمات کلیدی
D-galacturonic acidPDAASADTTqRT-PCRcDNA - cDNAComplementary DNA - DNA تکمیلیDNAse - DNAasel-ascorbate - L-آسکورباتl-Galactose - l-گالاکتوزAscorbic acid - آسکوربیک اسیدPhotodiode array detector - آشکارساز آرایه PhotodiodeAntioxidant - آنتی اکسیدانGene expression - بیان ژنdithiothreitol - دیتیوتریتولquantitative reverse transcription polymerase chain reaction - واکنش زنجیره ای پلی مراز رونویسی معکوسvitamin C - ویتامین سیhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
Citrus fruits are highly consumed worldwide and represent one of the most important sources of ascorbic acid (AsA). However, information about the molecular mechanisms regulating AsA accumulation in Citrus fruit and the effects of environmental factors is scarce. In this study we have investigated the effect of fruit shading on AsA content and the expression of AsA biosynthetic, degrading and recycling genes in fruits of different Citrus species. Immature-green fruits were covered at the end of the cell enlargement phase and AsA concentration in the flavedo declined and remained at low levels as compared with light-exposed fruits. Fruit shading marginally altered the expression of genes from the l-galactose pathway and this effect was variable in the four Citrus species. However, specific isoforms (GalUR8 or GalUR12) from the l-galacturonic acid pathway were significantly repressed paralleling the reduction in AsA concentration. No significant effect of shading was detected in transcription of genes of the myo-inositol and l-gulose pathways as well as recycling and degradation. Collectively, results indicate that light avoidance inhibited accumulation of AsA in the flavedo of Citrus fruits and suggest that the l-galacturonic acid pathway has a relevant contribution to AsA content in this tissue.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 231, February 2015, Pages 138-147
Journal: Plant Science - Volume 231, February 2015, Pages 138-147
نویسندگان
Joanna Lado, Enriqueta Alós, MarÃa Jesús Rodrigo, Lorenzo ZacarÃas,