کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8358312 | 1542065 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phytoalexin biosynthesis genes are regulated and involved in plant response to Ralstonia solanacearum infection
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کلمات کلیدی
Ralstonia solanacearum4-methylumbelliferyl β-D-glucuronide4-MUGElongation factor 1APTIT3SSEF1aGluAPAMPX-GlucDPIEASTPsGUSPAMPsTRVPCCGFPROS - ROSβ-glucuronidase - β-گلوکورونیدازHydrogen peroxide - آب اکسیژنهETI - اتیEthylene - اتیلنSalicylic acid - اسید سالیسیلیکmicrobe-associated molecular patterns - الگوهای مولکولی مرتبط با میکروبpathogen-associated molecular patterns - الگوهای مولکولی مرتبط با پاتوژنstandard deviation - انحراف معیارVIGS - ایدزPAMP-triggered immunity - ایمنی PAMPEffector-triggered immunity - ایمنی عامل اثرTerpene synthase - ترپن سنتازstandard error - خطای استانداردdigoxigenin - دیگوکسین ژنdays post inoculation - روز بعد از تلقیحType III secretion system - سیستم ترشحی نوع IIIDIG - شماMAMPS - مامس هاH2O2 - هیدروژن پراکسیدBacterial wilt - ویروس باکتریاییTobacco rattle virus - ویروس تنباکوvirus-induced gene silencing - ویروس ناشی از خاموش شدن ژنPAD - پدGreen fluorescence protein - پروتئین فلورسانس سبزPathogenesis-related gene - ژن مربوط به پاتوژنزReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Phytoalexin biosynthesis genes are regulated and involved in plant response to Ralstonia solanacearum infection Phytoalexin biosynthesis genes are regulated and involved in plant response to Ralstonia solanacearum infection](/preview/png/8358312.png)
چکیده انگلیسی
Genes encoding phytoalexin biosynthesis enzymes are transcriptionally regulated and are required for defense against fungi and oomycetes. Here, we studied the regulation of tobacco 5-epi-aristolochene synthase 4 (EAS4) promoters on bacterial infection and investigated the roles of tomato and Arabidopsis phytoalexin biosynthesis genes in defense against pathogenic bacteria. Our results showed that the Nicotiana glutinosa EAS4 (NgEAS4) promoter was significantly induced by treatments with several bacteria in treated and systemic leaves of transgenic plants. This promoter was also partially induced by treatments with type-III secretion-deficient mutants and total lysate of R. solanacearum (Rs), revealing that both bacterial pathogen-associated molecular patterns (PAMPs) and effectors are involved in R. solanacearum-induced local and systemic activation of the NgEAS4 promoter. Furthermore, the absence of a cis-element GT-1 box in the NgEAS4 promoter abolished systemic activation in non-treated leaves, whereas disruption of the GT-1 box of the N. tabacum EAS4 (NtEAS4) promoter led to constitutive expression. Moreover, silencing of tomato sesquiterpene synthase genes and disruption of the key gene PAD3 for Arabidopsis camalexin biosynthesis resulted in decreased tolerance to R. solanacearum. These results together, reveal the varied function of GT-1 boxes in regulating tobacco EAS4 promoters and the involvement of phytoalexin-biosythesis genes in plant defense against R. solanacearum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 224, July 2014, Pages 86-94
Journal: Plant Science - Volume 224, July 2014, Pages 86-94
نویسندگان
Yu-Mei Lin, Shu-Lein Shih, Wan-Chi Lin, Jia-Wei Wu, Ya-Ting Chen, Chi-Ying Hsieh, Li-Ching Guan, Lu Lin, Chiu-Ping Cheng,