کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8359442 1542293 2018 22 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Facilitation of yeast-lethal membrane protein production by detoxifying with GFP tagging
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Facilitation of yeast-lethal membrane protein production by detoxifying with GFP tagging
چکیده انگلیسی
Recombinant techniques for target protein production have been rapidly established and widely utilised in today's biological research. Nevertheless, methods for membrane protein production have yet to be developed, since membrane proteins generally tend to be expressed at low levels, easily aggregated, and/or even toxic to their host cells. Here we report that a GFP-tagging technique can be applied for the stable production of membrane proteins that are toxic to their host cells when overexpressed, paving the way for future advances in membrane protein biochemistry and drug development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 148, August 2018, Pages 40-45
نویسندگان
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