کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8383980 1543461 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Variable reproducibility in genome-scale public data: A case study using ENCODE ChIP sequencing resource
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Variable reproducibility in genome-scale public data: A case study using ENCODE ChIP sequencing resource
چکیده انگلیسی
Genome-wide data is accumulating in an unprecedented way in the public domain. Re-mining this data shows great potential to generate novel hypotheses. However this approach is dependent on the quality (technical and biological) of the underlying data. Here we performed a systematic analysis of chromatin immunoprecipitation (ChIP) sequencing data of transcription and epigenetic factors from the encyclopaedia of DNA elements (ENCODE) resource to demonstrate that about one third of conditions with replicates show low concordance between replicate peak lists. This serves as a case study to demonstrate a caveat concerning genome-wide analyses and highlights a need to validate the quality of each sample before performing further associative analyses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 589, Issue 24, Part B, 21 December 2015, Pages 3866-3870
نویسندگان
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