کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8387443 | 1543893 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of Helja, an extracellular jacalin-related protein from Helianthus annuus: Insights into the relationship of this protein with unconventionally secreted lectins
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کلمات کلیدی
ORFRT-PCRApoplastEST - استUnconventional secretion - ترشح غیر متعارفcoding sequence - توالی کدگذاریExpressed Sequence Tag - تکرار اظهار نظرopen reading frame - قاب خواندن بازLectin - لکتینJacalin-related lectin - لکتین مرتبط با ژاکالینreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسExtracellular proteins - پروتئین های غیر سلولیCdS - کادمیم سولفید، سولفید کادمیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Jacalin-related lectins (JRLs) encompass cytosolic, nuclear and vacuolar members displaying the jacalin domain in one or more copies or in combination with unrelated domains. Helianthus annuus jacalin (Helja) is a mannose-specific JRL previously identified in the apoplast of Helianthus annuus seedlings, and this protein has been proposed to follow unconventional secretion. Here, we describe the full-length Helja cDNA sequence, which presents a unique jacalin domain (merolectin) and the absence of a signal peptide, confirming that the protein cannot follow the classical ER-dependent secretory pathway. Helja mRNA is present in seeds, cotyledons, roots and hypocotyls, but no transcripts were detected in the leaves. Searches for sequence similarity showed that Helja is barely similar to other JRLs present in H. annuus databases and less than 45% identical to other monocot or dicot JRLs. Strikingly, most of the merolectins recovered through data mining using Helja as a query were predicted as apoplastic, although most of these proteins lack the signal peptide required for classical secretion. Thus, Helja is the first bait identified to recover putative unconventionally secreted lectins. Because the recovered JRLs are widely distributed among the plant kingdom, an as yet unknown role for jacalin lectins in the apoplast is emerging.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 183, 1 July 2015, Pages 144-153
Journal: Journal of Plant Physiology - Volume 183, 1 July 2015, Pages 144-153
نویسندگان
Marcela Pinedo, Facundo Orts, André de Oliveira Carvalho, Mariana Regente, Julia Ribeiro Soares, Valdirene Moreira Gomes, Laura de la Canal,