کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8396556 | 1544153 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of health status of horses immunized with snake venom and montanide adjuvants, IMS 3012 (nanoparticle), ISA 206 and ISA 35 (emulsion based) during polyvalent snake antivenom production: Hematological and biochemical assessment
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کلمات کلیدی
NSSIL6IL1GLBTECLD50TLCALBSnake antivenom - antivenom مارAlbumin - آلبومینALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتinterleukin-6 - اینترلوکین ۶interleukin-1 - اینترلوکین-1bil - بودBilirubin - بیلیروبینtotal leukocyte count - تعداد کل لکوسیت هاtumor necrosis factor-alpha - تومور نکروز عامل آلفاRussell's viper venom - جاذبه روسل راسPacked cell volume - حجم سلول بسته بندی شدهBUN - خوبnormal saline solution - راه حل نرمال سالینcobra venom - سم کبراTNF-α - فاکتور نکروز توموری آلفاmedian lethal dose - میانگین دوز مرگبارblood urea nitrogen - نیتروژن اوره خونHemoglobin - هموگلوبینPCV یا Pneumococcal conjugate vaccine - واکسن کونژوگه پنوموکوکCreat - کراتcreatinine - کراتینینtotal protein - کل پروتئینGlobulin - گلوبولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Several biochemical and hematological changes in horses are observed during production of snake antivenom. Although conventional adjuvants like Freund's (Complete and Incomplete) are good immunopotentiators, they produce considerable local reactions in animals. Variety of commercial adjuvants, like montanide adjuvants, having high immunopotentiation and showing lesser side effects are available. The prime objective during antivenom production is to strike a balance between safety of immunized horses and efficacy of the product. In our earlier work, efficacy of montanide group of adjuvants in antivenom production has already been established. The aim of the present work was to assess the safety parameters in horses, viz.: biochemical and hematological, during production of snake antivenom. In the present study, 33 new horses were randomly divided into four groups and hyperimmunized using mixture of snake venoms, viz.: Cobra venom, Russell's viper venom, Krait venom and Echis venom along with montanide adjuvants, IMS 3012, ISA 206, ISA 35 and Incomplete Freund's adjuvant as a control adjuvant; through subcutaneous route at intervals of two weeks. During the immunization period, biochemical and hematological parameters were monitored at 0th, 14th, 21st, 30th and 42nd weeks. The mean hemoglobin values dropped slightly during initial immunization but subsequently regained to normal levels. The mean serum total protein values and globulin levels showed an increment in all the four groups, compared to day zero, vice-versa a slight drop was observed in albumin levels. No significant changes were observed in serum creatinine, bilirubin, alkaline phosphatase and blood urea nitrogen values. Finally, we conclude that montanide adjuvants could be a safer alternative to the conventional adjuvants for primary phase of immunization in antivenom production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 82, May 2014, Pages 83-92
Journal: Toxicon - Volume 82, May 2014, Pages 83-92
نویسندگان
A.B. Waghmare, N.C. Salvi, R.L. Deopurkar, P.A. Shenoy, J.M. Sonpetkar,