کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8397444 | 1544163 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative immunochemical characteristics of botulinum neurotoxin type A and its associated proteins
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کلمات کلیدی
PBSTNAPSNBPBoNTTBSTABTSIgGPBSBoNT/A - BoNT / AImmunogenicity - ایمنی زاییimmunoglobulin G - ایمونوگلوبولین GBotulinum - بوتولینومEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاPhosphate buffered saline - فسفات بافر شورBotulinum neurotoxin - نوروتوکسین بوتولینومbotulinum neurotoxin type A - نوع نوروتوکسین بوتولینوم نوع AClostridium - کلوستریدیوم Complex - کمپلکس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
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چکیده انگلیسی
Clostridium botulinum strains secrete their neurotoxins (BoNT) along with a group of neurotoxin-associated proteins (NAPs) that enhance the oral toxicity and provide protection to the neurotoxin against acidity, temperature and proteases in the G.I. tract. A major component of NAPs is Hn-33, a 33Â kDa protein, which is also protease resistant and strongly protects BoNT. The complex form of BoNT/A is used as a commercial therapeutic formulation against many neuromuscular disorders and for cosmetic purposes. Immune response against this formulation could hinder its long-term use; therefore, it is important to characterize the immunological properties of the associated proteins. This study aims to understand the immunological reactivity of BoNT/A complex, BoNT, NAPs, and Hn-33 through a series of competitive enzyme-linked immunosorbent assays (ELISA). The results indicated that BoNT/A complex competed 6 times more with complex antibodies compared to the neurotoxin confirming that the higher immunogenicity of BoNT/A complex was indeed a result of the associated proteins with the neurotoxin complex. While the nearly identical immuno-reactivity of BoNT/A complex and Hn-33 with Hn-33 antibodies indicated that the reactivity was due to the higher immunogenicity not the abundance of Hn-33 in the complex. Both the ELISA and immuno-blot results implied that Hn-33 is primarily responsible for eliciting the antibody response in BoNT/A complex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 72, 15 September 2013, Pages 126-132
Journal: Toxicon - Volume 72, 15 September 2013, Pages 126-132
نویسندگان
Anne-Marie Bryant, Shuowei Cai, Bal Ram Singh,