کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8417204 | 1545677 | 2016 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative genomics analysis of mononuclear phagocyte subsets confirms homology between lymphoid tissue-resident and dermal XCR1+ DCs in mouse and human and distinguishes them from Langerhans cells
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کلمات کلیدی
FDRmoDCsGSEALCSDMPSXCR1nESdDCsPCA - PCABioinformatics - بیوانفورماتیک Gene Set Enrichment Analysis - تجزیه و تحلیل غنی سازی مجموعه ژنیPrincipal component analysis - تحلیل مولفههای اصلی یا PCADendritic cells - سلول های دندریتیکLangerhans cells - سلول های لانگرهانسMonocyte-derived dendritic cells - سلولهای دندریتیک مونوسیتDermal dendritic cells - سلولهای دندریتیک پوستmononuclear phagocytes - فاگوسیت های تک هسته ایMacrophages - ماکروفاژها،درشت خوارهاMonocytes - مونوسیتهاMAC - مکfalse discovery rate - میزان کشف کاذبMPs - نمایندگان مجلسnormalized enrichment score - نمره غنی سازی نرمال شدهSkin - پوستComparative genomics - ژنومیک تطبیقی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Dendritic cells (DC) are mononuclear phagocytes which exhibit a branching (dendritic) morphology and excel at naïve T cell activation. DC encompass several subsets initially identified by their expression of cell surface molecules and later shown to possess distinct functions. DC subset differentiation is orchestrated by transcription factors, growth factors and cytokines. Identifying DC subsets is challenging as very few cell surface molecules are uniquely expressed on any one of these cell populations. There is no standard consensus to identify mononuclear phagocyte subsets; varying antigens are employed depending on the tissue and animal species studied and between laboratories. This has led to confusion in how to accurately define and classify DCs across tissues and between species. Here we report a comparative genomics strategy that enables universal definition of DC and other mononuclear phagocyte subsets across species. We performed a meta-analysis of several public datasets of human and mouse mononuclear phagocyte subsets isolated from blood, spleen, skin or cutaneous lymph nodes, including by using a novel and user friendly software, BubbleGUM, which generates and integrates gene signatures for high throughput gene set enrichment analysis. This analysis demonstrates the equivalence between human and mouse skin XCR1+ DCs, and between mouse and human Langerhans cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 432, May 2016, Pages 35-49
Journal: Journal of Immunological Methods - Volume 432, May 2016, Pages 35-49
نویسندگان
Sabrina Carpentier, Thien-Phong Vu Manh, Rabie Chelbi, Sandrine Henri, Bernard Malissen, Muzlifah Haniffa, Florent Ginhoux, Marc Dalod,