کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8417895 | 1545706 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protein expression profiling by antibody array analysis with use of dried blood spot samples on filter paper
ترجمه فارسی عنوان
تجزیه و تحلیل بیان پروتئین با استفاده از تجزیه و تحلیل آرایه آنتی بادی با استفاده از نمونه های خونی خالی در کاغذ فیلتر
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
MDCNAP-2DBSSRegulated upon Activation, Normal T cell Expressed and SecretedIGFBP-1MCP-1PKUMFIPDGF-BBEGFphenylketonuria - فنیل کتونوریBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز Antibody array - آرایه آنتی بادیinterleukin-1 alpha - اینترلوکین-1 آلفاSerum - سرمepidermal growth factor - عامل رشد اپیدرمیBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزRANTES - مطالبmean fluorescent intensity - میانگین شدت فلورسنتBiomarkers - نشانگر زیستی یا بیومارکرProteomics - پروتئومیکسInsulin-like growth factor binding protein 1 - پروتئین اتصال دهنده فاکتور رشد مانند انسولین 1monocyte chemoattractant protein-1 - پروتئین شیمیایی monocyte chemoattractant-1Macrophage-derived chemokine - کیموکین مشتق شده از ماکروفاژ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
Dried blood spot samples (DBSS) on filter paper offer several advantages compared to conventional serum/plasma samples: they do not require any phlebotomy or separation of blood by centrifugation; they are less invasive; they allow sample stability and shipment at room temperature; and they pose a negligible risk of infection with blood-borne viruses, such as HIV, HBV and HCV, to those who handle them. Therefore dried blood spot samples (DBSS) on filter paper can be a quick, convenient and inexpensive means of obtaining blood samples for biomarker discovery, disease screening, diagnosis and treatment monitoring in non-hospitalized, public health settings. In this study, we investigated for the first time the potential application of dried blood spot samples (DBSS) in protein expression profiling using antibody array technology. First, optimal conditions for array assay performance using dried blood spot samples (DBSS) was established, including sample elution buffer, elution time, elution temperature and assay blocking buffer. Second, we analyzed dried blood spot samples (DBSS) using three distinct antibody array platforms, including sandwich-based antibody arrays, quantitative antibody arrays and biotin-label-based antibody arrays. In comparison with paired serum samples, detection of circulating proteins in dried blood spot samples (DBSS) correlated well for both low- and high-abundance proteins on all three antibody array platforms. In conclusion, our study strongly indicates the novel application of multiplex antibody array platforms to analyze dried blood spot samples (DBSS) on filter paper represents a viable, cost-effective method for protein profiling, biomarker discovery and disease screening in a large, population-based survey.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 403, Issues 1â2, 31 January 2014, Pages 79-86
Journal: Journal of Immunological Methods - Volume 403, Issues 1â2, 31 January 2014, Pages 79-86
نویسندگان
Weidong Jiang, Ying Qing Mao, Ruochun Huang, Chaohui Duan, Yun Xi, Kai Yang, Ruo-Pan Huang,