کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8418710 | 1545727 | 2012 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
All major prion types recognised by a multiplex immunofluorometric assay for disease screening and confirmation in sheep
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: All major prion types recognised by a multiplex immunofluorometric assay for disease screening and confirmation in sheep All major prion types recognised by a multiplex immunofluorometric assay for disease screening and confirmation in sheep](/preview/png/8418710.png)
چکیده انگلیسی
Furthermore, analysis of serially diluted samples indicated that the assay provided a similar level of sensitivity for atypical scrapie as that found using a well established commercial test. Unexpectedly, 9A2 binding to CH1641 PrPres was reduced by 2.1 fold both for experimental CH1641 and CH1641-like scrapie when compared with BSE, suggesting that major cleavage of the N-terminus occurs further towards the C-terminus in CH1641 than in BSE. The ratios of 12B2/94B4 and 9A2/94B4 were similar between experimental CH1641 and CH1641-like cases, although two CH1641-like subjects displayed slightly elevated ratios of both 12B2/94B4 and 9A2/94B4. To verify this finding for PrPres, mass spectrometry based quantification was used to determine the absolute abundance of the peptides associated with all three antibody binding regions. There was a 2.2 fold reduction of peptides containing the 9A2 epitope for experimental CH1641 PrPres in comparison to BSE PrPres. Observation of reduced PrPres may serve as a new marker for CH1641. This mIFMA may thus provide the basis for simplified TSE diagnosis with capability for simultaneous screening and differential diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 380, Issues 1â2, 29 June 2012, Pages 30-39
Journal: Journal of Immunological Methods - Volume 380, Issues 1â2, 29 June 2012, Pages 30-39
نویسندگان
Yue Tang, Adriana Gielbert, Jorg G. Jacobs, Thierry Baron, Olivier Andreoletti, Takashi Yokoyama, Jan P.M. Langeveld, Maurice J. Sauer,