کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8421534 | 1545928 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
In this study, a simple and rapid method was developed to transform protein A producing Staphylococcus aureus cells into red color and red fluorescent nanobioparticles, which were homogeneous, dispersive and relatively stable with a uniform size of 800 nm. The method consists of reaction with a monotetrazolium redox dye at 25 °C for 15 min and heat inactivation at 65 °C for 30 min. This method provided the first S. aureus nanobioparticles with the dual property of red color and red fluorescence. Attributed to the IgG binding site known as protein A on their surface, the nanobioparticles could be used as vectors for immunoassays of many bacteria and viruses. Coagglutination test of Escherichia coli O157:H7 observed by naked eyes showed that the detection limitation of the nanobioprobes was 1 â 106 CFU/ml, which was about 100 times more sensitive than the natural uncolored S. aureus bioprobes. Red fluorescence detection and analysis of the coagglutination product by a microplate reader lowered the detection limit to 2.5 â 104 CFU/ml.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 115, August 2015, Pages 47-53
Journal: Journal of Microbiological Methods - Volume 115, August 2015, Pages 47-53
نویسندگان
Wei Hu, Yun Zhang, Hang Yang, Junping Yu, Hongping Wei,