کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8421534 1545928 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection
چکیده انگلیسی
In this study, a simple and rapid method was developed to transform protein A producing Staphylococcus aureus cells into red color and red fluorescent nanobioparticles, which were homogeneous, dispersive and relatively stable with a uniform size of 800 nm. The method consists of reaction with a monotetrazolium redox dye at 25 °C for 15 min and heat inactivation at 65 °C for 30 min. This method provided the first S. aureus nanobioparticles with the dual property of red color and red fluorescence. Attributed to the IgG binding site known as protein A on their surface, the nanobioparticles could be used as vectors for immunoassays of many bacteria and viruses. Coagglutination test of Escherichia coli O157:H7 observed by naked eyes showed that the detection limitation of the nanobioprobes was 1 ∗ 106 CFU/ml, which was about 100 times more sensitive than the natural uncolored S. aureus bioprobes. Red fluorescence detection and analysis of the coagglutination product by a microplate reader lowered the detection limit to 2.5 ∗ 104 CFU/ml.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 115, August 2015, Pages 47-53
نویسندگان
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