کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8426247 | 1546051 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pig StAR: mRNA expression and alternative splicing in testis and Leydig cells, and association analyses with testicular morphology traits
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کلمات کلیدی
qRT-PCRTSGEDSSVS3β-Hydroxysteroid dehydrogenaseCYP17A1LCSCYP11A1HWESSCscytochrome P450 family 11 subfamily A member 1SCsPICTLGCytochrome P450 17A1HSD17B3SNPsInDelHsd3beffective allele numbers - اعداد موثر آللStructural variants - انواع ساختاریgenetic variants - انواع ژنتیکیHardy-Weinberg equilibrium - تعادل هاردی-وینبرگAlternative splicing - جابجایی جایگزینstandard error - خطای استانداردPig - خوکinsertion/deletion - درج / حذفStar - ستارهSpermatogonial stem cells - سلول های بنیادی اسپرماتوگونالیLeydig cells - سلولهای LeydigSertoli cells - سلولهای Sertolistart - شروع کنReproductive Traits - صفات تولید مثلpolymorphism information content - محتوای اطلاعات پلی مورفیسمHeterozygosity - هتروزیگوزیتهHomozygosity - هموسیگوییquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیSteroidogenic acute regulatory protein (StAR) - پروتئین حاد استروئیدوژنیک (StAR)Steroidogenic acute regulatory protein - پروتئین حاکم استروئیدوژنیک حادSingle nucleotide polymorphisms - پلیمورفیسم تک نوکلئوتیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Pig StAR: mRNA expression and alternative splicing in testis and Leydig cells, and association analyses with testicular morphology traits Pig StAR: mRNA expression and alternative splicing in testis and Leydig cells, and association analyses with testicular morphology traits](/preview/png/8426247.png)
چکیده انگلیسی
Steroidogenic acute regulatory protein (StAR), primarily expressed in Leydig cells (LCs) in the mammalian testes, is essential for testosterone biosynthesis and male fertility. However, no previous reports have explored the expression profiles, alternative splicing and genetic variations of StAR gene in pig. The aim of current study was to explore the expression profiles in different tissues and different types of testicular cells (LCs; spermatogonial stem cells, SSCs; Sertoli cells, SCs), to identify different splice variants and their expression levels, as well as to detect the indel polymorphism in pig StAR gene. Expression analysis results revealed that StAR was widely expressed in all tested tissues and the expression level in testis was significantly higher than that in other tissues (Pâ¯<â¯0.01); among different types of testicular cells, the StAR mRNA expression level was significantly higher in LCs than others (Pâ¯<â¯0.05). Furthermore, three splice variants, StAR-a, StAR-b and StAR-c, were first found in pig. Further study showed StAR-a was highly expressed in both testis and LCs when compared with other variants (Pâ¯<â¯0.01), suggesting StAR-a was the primary variant at StAR gene post-transcription and may facilitate the combination and transportation of cholesterol with StAR. In addition, a 5-bp duplicated deletion (NC_010457.5:g.5524-5528 delACTTG) was verified in the porcine StAR gene, which was closely related to male testicular morphology traits (Pâ¯<â¯0.05), and we speculated that the allele “D” of StAR gene might be a positive allele. Briefly, the current findings suggest that StAR and StAR-a play imperative roles in male fertility and the 5-bp indel can be a potential DNA marker for the marker-assisted selection in boar.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 118, 15 September 2018, Pages 46-56
Journal: Theriogenology - Volume 118, 15 September 2018, Pages 46-56
نویسندگان
Yanghai Zhang, Yang Cui, Xuelian Zhang, Yimin Wang, Jiayang Gao, Ting Yu, Xiaoyan Lv, Chuanying Pan,