کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8460517 | 1548986 | 2017 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thermogenesis, fatty acid synthesis with oxidation, and inflammation in the brown adipose tissue of ob/ob (â/â) mice
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کلمات کلیدی
FABP4subcutaneous WATTNFalphaTC10GLUT4MCP1IBATCD36carnitine palmitoyltransferase 1bCpt1bBATFASSREBP1cperoxisome proliferator-activated receptor gamma coactivator 1-alpha - 1-آلفا کوآتیواتور گاما گیرنده فعال پرولیسینز فعالPLIN2 - gas2Ucp1 - UCP1Akt - آکتfatty acid synthase - اسید چرب سنتازinflammation - التهاب( توروم) interleukin - اینترلوکینWhite adipose tissue - بافت چربی سفیدbrown adipose tissue - بافت چربی قهوه ایSWAT - تحلیل سواتThermogenesis - ترموژنزtumor necrosis factor alpha - تومور نکروز عامل آلفاBody mass - جرم بدنcluster of differentiation 36 - خوشه تمایز 36Molecular biology - زیستشناسی مولکولیObesity - مرض چاقیGlucose transporter type 4 - نوع حمل گلوکز 4wild-type - نوع وحشیEnergy expenditure - هزینه انرژیmonocyte chemotactic protein 1 - پروتئین chemotactic monocyte 1fatty acid binding protein 4 - پروتئین اتصال دهنده اسید چرب 4uncoupling protein 1 - پروتئین جدا سازی 1protein kinase B - پروتئین کیناز Bperilipin 2 - پریلیپین 2WAT - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Thermogenesis, fatty acid synthesis with oxidation, and inflammation in the brown adipose tissue of ob/ob (â/â) mice Thermogenesis, fatty acid synthesis with oxidation, and inflammation in the brown adipose tissue of ob/ob (â/â) mice](/preview/png/8460517.png)
چکیده انگلیسی
Brown adipose tissue (BAT) is specialized in heat production, but its metabolism in ob/ob mice is still a matter of debate. We aimed to verify ob/ob mice BAT using C57Bl/6 male mice (as the wild-type, WT) and leptin-deficient ob/ob mice (on the C57Bl/6 background strain), at three months of age (n = 10/group). At euthanasia, animals had their interscapular BAT weighed, and prepared for analysis (Western blot, and RT-qPCR). In comparison with the WT group, the ob/ob group showed reduced thermogenic signaling markers (gene expression of beta 3-adrenergic receptor, beta3-AR; PPARgamma coactivator 1 alpha, PGC1alpha, and uncoupling protein 1, UCP1). The ob/ob group also showed impaired gene expression for lipid utilization (perilipin was increased, while other markers were diminished: carnitine palmitoyltransferase-1b, CPT-1b; cluster of differentiation 36, CD36; fatty acid binding protein 4, FABP4; fatty acid synthase, FAS, and sterol regulatory element-binding protein 1c, SREBP1c), and altered protein expression of insulin signaling (diminished pAKT, TC10, and GLUT-4). Lastly, the ob/ob group showed increased gene expression of markers of inflammation (interleukin 1 beta, IL-1beta; IL-6, tumor necrosis factor alpha, TNFalpha; and monocyte chemotactic protein-1, MCP-1). In conclusion, the ob/ob mice have decreased thermogenic markers associated with reduced gene expression related to fatty acid synthesis, mobilization, and oxidation. There were also alterations in insulin signaling and protein and gene expressions of inflammation. The findings suggest that the lack of substrate for thermogenesis and the local inflammation negatively regulated thermogenic signaling in the ob/ob mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 210, March 2017, Pages 44-51
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 210, March 2017, Pages 44-51
نویسندگان
Fabiane Ferreira Martins, Thereza Cristina Lonzetti Bargut, Marcia Barbosa Aguila, Carlos Alberto Mandarim-de-Lacerda,