کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8473755 | 1550409 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Misexpression of Tbx18 in cardiac chambers of fetal mice interferes with chamber-specific developmental programs but does not induce a pacemaker-like gene signature
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کلمات کلیدی
GJCSHOX2LBHPitx2TAGLNIRESHprtT-boxAVNVSDSANHCNDAPITNNI3GAPDHChIP-SeqNppaGFPSCN5ATbx - TBXChromatin immunoprecipitation-sequencing - ایمن سازی کروماتین-توالیTransgelin - ترانژلینfold change - تغییر در برابرembryonic day - روز جنینیpostnatal day - روز پس از زایمانinternal ribosomal entry site - سایت ورودی ریبوزوم داخلیPaired-like homeodomain transcription factor 2 - فاکتور رونویسی هومیوودینام 2 مانندVentricular septal defect - نقص دیواره بین بطنیatrial septal defects - نقص سپتوم دهلیزیASD - نقص سپتوم یا دیوارهی دهلیزیhypoxanthine guanine phosphoribosyl transferase - هیپوکسانتین گوانین فسفریبوسیل ترانسفرازpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازaction potential - پتانسیل عمل green fluorescent protein - پروتئین فلورسنت سبزPeriostin - پریستینPostn - پست پستconnexin - کنسکسینSinoatrial node - گره SinoatrialAtrioventricular node - گره اتریواستروییglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Misexpression of Tbx18 in cardiac chambers of fetal mice interferes with chamber-specific developmental programs but does not induce a pacemaker-like gene signature Misexpression of Tbx18 in cardiac chambers of fetal mice interferes with chamber-specific developmental programs but does not induce a pacemaker-like gene signature](/preview/png/8473755.png)
چکیده انگلیسی
Initiation of cardiac excitation depends on a specialized group of cardiomyocytes at the venous pole of the heart, the sinoatrial node (SAN). The T-box transcription factor gene Tbx18 is expressed in the SAN myocardium and is required for formation of a large portion of the pacemaker. Previous studies suggested that Tbx18 is also sufficient to reprogram ventricular cardiomyocytes into SAN cells in rat, guinea-pig and pig hearts. To evaluate the consequences of misexpression of Tbx18 for imposing a nodal phenotype onto chamber myocardial cells in fetal mice, we used two independent conditional approaches with chamber-specific cre driver lines and an HprtTbx18 misexpression allele. Myh6-Cre/+;HprtTbx18/y mice developed dilated atria with thickened walls, reduced right ventricles and septal defects that resulted in reduced embryonic and post-natal survival. Tagln-Cre/+;HprtTbx18/y mice exhibited slightly smaller hearts with rounded trabeculae that supported normal embryonic survival. Molecular analyses showed that the SAN gap junction and ion channel profile was not ectopically induced in chamber myocardium but the working myocardial gene program was partially inhibited in atria and ventricles of both misexpression models. Left atrial expression of Pitx2 was strongly repressed in Myh6-Cre/+;HprtTbx18/y embryos. We conclude that exclusion of Tbx18 expression from the developing atria and (right) ventricle is important to achieve normal cardiac left-right patterning and myocardial differentiation, and that Tbx18 is not sufficient to induce full SAN differentiation of chamber cardiomyocytes in fetal mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 97, August 2016, Pages 140-149
Journal: Journal of Molecular and Cellular Cardiology - Volume 97, August 2016, Pages 140-149
نویسندگان
Franziska Greulich, Mark-Oliver Trowe, Andreas Leffler, Carsten Stoetzer, Henner F. Farin, Andreas Kispert,