کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8479111 | 1551285 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lack of presynaptic interaction between glucocorticoid and CB1 cannabinoid receptors in GABA- and glutamatergic terminals in the frontal cortex of laboratory rodents
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کلمات کلیدی
MifepristoneVGATVGLUT1DAGLα[3H]GABAGPCR4-APdpm3RspresynapticDEXAWIN55212-2HEPESPFC4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid4-aminopyridine - 4-آمینوپیریدینCB1 cannabinoid receptor - CB1 گیرنده کانابینوئیدDMSO - DMSOsodium dodecyl sulphate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدARRIVE - برگرفته ازvesicular GABA transporter - حامل ویسکوزر GABAvesicular glutamate transporter 1 - حمل کننده گلوتامات وزیکولار 1Dexamethasone - دگزامتازونDimethylsulfoxide - دیمتیل سولفواکسیدdisintegration per minute - فروپاشی در هر دقیقهprefrontal cortex - قشر prefrontalfrontal cortex - قشر جلوییHPA - میلی بار یا هکتوپاسکالhypothalamo-pituitary-adrenal - هیپوتالامو-هیپوفیز-آدرنالWestern blotting - وسترن بلاتینگWIN - پیروزیCorticosterone - کورتیکوسترونGABA - گاباglutamate - گلوتاماتglucocorticoid receptor - گیرنده گلوکوکورتیکوئیدG protein-coupled receptor - گیرندههای جفتشونده با پروتئین جی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Corticosteroid and endocannabinoid actions converge on prefrontocortical circuits associated with neuropsychiatric illnesses. Corticosteroids can also modulate forebrain synapses by using endocannabinoid effector systems. Here, we determined whether corticosteroids can modulate transmitter release directly in the frontal cortex and, in doing so, whether they affect presynaptic CB1 cannabinoid receptor- (CB1R) mediated neuromodulation. By Western blotting of purified subcellular fractions of the rat frontal cortex, we found glucocorticoid receptors (GcRs) and CB1Rs enriched in isolated frontocortical nerve terminals (synaptosomes). CB1Rs were predominantly presynaptically located while GcRs showed preference for the post-synaptic fraction. Additional confocal microscopy analysis of cortical and hippocampal regions revealed vesicular GABA transporter-positive and vesicular glutamate transporter 1-positive nerve terminals endowed with CB1R immunoreactivity, apposing GcR-positive post-synaptic compartments. In functional transmitter release assay, corticosteroids, corticosterone (0.1-10 microM) and dexamethasone (0.1-10 microM) did not significantly affect the evoked release of [3H]GABA and [14C]glutamate in superfused synaptosomes, isolated from both rats and mice. In contrast, the synthetic cannabinoid, WIN55212-2 (1 microM) diminished the release of both [3H]GABA and [14C]glutamate, evoked with various depolarization paradigms. This effect of WIN55212-2 was abolished by the CB1R neutral antagonist, O-2050 (1 microM), and was absent in the CB1R KO mice. CB2R-selective agonists did not affect the release of either neurotransmitter. The lack of robust presynaptic neuromodulation by corticosteroids was unchanged upon either CB1R activation or genetic inactivation. Altogether, corticosteroids are unlikely to exert direct non-genomic presynaptic neuromodulation in the frontal cortex, but they may do so indirectly, via the stimulation of trans-synaptic endocannabinoid signaling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 90, November 2015, Pages 72-84
Journal: Neurochemistry International - Volume 90, November 2015, Pages 72-84
نویسندگان
Rafael M. Bitencourt, Alán Alpár, Valentina Cinquina, Samira G. Ferreira, Bárbara S. Pinheiro, Cristina Lemos, Catherine Ledent, Reinaldo N. Takahashi, Fernando J. Sialana, Gert Lubec, Rodrigo A. Cunha, Tibor Harkany, Attila Köfalvi,