کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8485584 | 1551743 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant M. bovis BCG expressing the PLD protein promotes survival in mice challenged with a C. pseudotuberculosis virulent strain
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
The aim of this study was to evaluate the survival of mice inoculated with M. bovis BCG Pasteur recombinant expressing the PLD protein and challenged with a C. pseudotuberculosis virulent strain. Four groups were immunized with a sterile 0.9% saline solution (G1), 106â¯CFU of M. bovis BCG Pasteur (G2), 106â¯CFU of M. bovis BCG/pld (G3) or 106â¯CFU of M. bovis BCG/pld with a booster with rPLD (G4) and challenged with 104 CFU of C. pseudotuberculosis MIC-6 strain. The highest survival rate of 88% was observed in G4, followed by 77% in G3 and 66% in G2. A significant statistical difference was observed in the levels of cytokines IFN-γ and IL-10 in vaccinated groups (G3 and G4) when compared with the control group (G1) (pâ¯<â¯0.05). The results seem promising as the recombinant vaccine elicited a cellular immune response and provided significant survival after a high virulent challenge.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 36, Issue 25, 14 June 2018, Pages 3578-3583
Journal: Vaccine - Volume 36, Issue 25, 14 June 2018, Pages 3578-3583
نویسندگان
Karen Silva Leal, Mara Thais de Oliveira Silva, Andréa de Fátima Silva Rezende, Francisco Silvestre Brilhante Bezerra, Karine Begnini, Fabiana Seixas, Tiago Collares, Odir Dellagostin, Ricardo Wagner Portela, Vasco Ariston de Carvalho Azevedo,