کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8485621 | 1551744 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Decreased accumulation of subgenomic RNA in human cells infected with vaccine candidate DEN4Î30 increases viral susceptibility to type I interferon
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Decreased accumulation of subgenomic RNA in human cells infected with vaccine candidate DEN4Î30 increases viral susceptibility to type I interferon Decreased accumulation of subgenomic RNA in human cells infected with vaccine candidate DEN4Î30 increases viral susceptibility to type I interferon](/preview/png/8485621.png)
چکیده انگلیسی
The NIH has developed live attenuated dengue virus (DENV) vaccine candidates by deletion of 30 nucleotides (Î30) from the untranslated region of the viral genome. Although this attenuation strategy has proven to be effective in generating safe and immunogenic vaccine strains, the molecular mechanism of attenuation is largely unknown. To examine the mediators of the observed attenuation phenotype, differences in translation efficiency, genome replication, cytotoxicity, and type I interferon susceptibility were compared between wild type parental DENV and DENVÎ30 attenuated vaccine candidates. We observed that decreased accumulation of subgenomic RNA (sfRNA) from the vaccine candidates in infected human cells causes increased type I IFN susceptibility and propose this as one of the of attenuation mechanisms produced by the 3â² UTR Î30 mutation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 36, Issue 24, 7 June 2018, Pages 3460-3467
Journal: Vaccine - Volume 36, Issue 24, 7 June 2018, Pages 3460-3467
نویسندگان
José Bustos-Arriaga, Gregory D. Gromowski, Konstantin A. Tsetsarkin, Cai-Yen Firestone, Tannya Castro-Jiménez, Alexander G. Pletnev, Leticia Cedillo-Barrón, Stephen S. Whitehead,