کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8486080 | 1551763 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Early immune responses and profiling of cell-mediated immunity-associated gene expression in response to rHVT-IBD vaccination
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Early immune responses and profiling of cell-mediated immunity-associated gene expression in response to rHVT-IBD vaccination Early immune responses and profiling of cell-mediated immunity-associated gene expression in response to rHVT-IBD vaccination](/preview/png/8486080.png)
چکیده انگلیسی
Infectious bursal disease (IBD) remains a major threat to the poultry industry. Recombinant herpesvirus of turkey (rHVT)-IBD vaccines have been successfully used to induce a protective immune response against IBD. However, the capacity for rHVT-IBD vaccines to induce early protection without detectable antibodies, and the underlying mechanisms mediating specific cell-mediated responses in the early stages following vaccination, have been poorly investigated. Therefore, in this study, specific pathogen-free (SPF) chickens were vaccinated with rHVT-IBD and T-cell subsets were analyzed. Both splenic and circulating CD8+ cell populations increased at 7â¯days postvaccination (dpv). Next, the expression of adaptive immunity-related genes was analyzed in the spleen and lung of rHVT-IBD-vaccinated chickens. Upregulation of CD8 expression was observed at 7â¯dpv. Interestingly, a parallel increase in the transcription of granzymes A and K was also detected from 7â¯dpv. To our knowledge, the latter result is the first to be reported, and it suggests that cytotoxic activity of CD8+ T lymphocytes is activated. In contrast, expression of the innate genes examined remained largely unchanged following vaccination. To further investigate the IBD virus (IBDV)-specific responses triggered by rHVT-IBD vaccination, vaccinated chickens were inoculated with an attenuated IBDV strain with the aim of restimulating induced immune responses in vivo. The expression profiles of various genes associated with adaptive immune responses were subsequently analyzed in lung, spleen, and bursa of Fabricius samples. Significant upregulation of CD4, CD8, perforin, and IFNγ expression were observed in the bursa samples 7â¯days postinoculation (dpi). In the lung, transcript levels of CD8, granzymes and perforin were also significantly higher in the rHVT-IBD-vaccinated chickens at 7â¯dpi, thereby suggesting that specific cellular immune responses were activated. Overall, these results support the hypothesis that stimulation of specific CD8+ cell-mediated immunity contributes to the response against IBDV in rHVT-IBD-vaccinated chickens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 36, Issue 5, 29 January 2018, Pages 615-623
Journal: Vaccine - Volume 36, Issue 5, 29 January 2018, Pages 615-623
نویسندگان
Fiona Ingrao, Fabienne Rauw, Mieke Steensels, Thierry van den Berg, Bénédicte Lambrecht,