کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8486615 | 1551774 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Attenuation and protective efficacy of Rift Valley fever phlebovirus rMP12-GM50 strain
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کلمات کلیدی
FBSORFi.p.RVFRVFVMOI - MERift Valley fever - تب ریفت درهminimum essential medium - حداقل حداقل مورد نیازfetal bovine serum - سرم جنین گاوopen reading frame - قاب خواندن بازMEM - مامانIntraperitoneal route - مسیر داخل چشمیi.m. - من هستم.plaque forming units - واحدهای تشکیل پلاکpfu - پفوmultiplicity of infection - چندین عفونت
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Arabian Peninsula that affects sheep, cattle, goats, camels, and humans. Effective vaccination of susceptible ruminants is important for the prevention of RVF outbreaks. Live-attenuated RVF vaccines are in general highly immunogenic in ruminants, whereas residual virulence might be a concern for vulnerable populations. It is also important for live-attenuated strains to encode unique genetic markers for the differentiation from wild-type RVFV strains. In this study, we aimed to strengthen the attenuation profile of the MP-12 vaccine strain via the introduction of 584 silent mutations. To minimize the impact on protective efficacy, codon usage and codon pair bias were not de-optimized. The resulting rMP12-GM50 strain showed 100% protective efficacy with a single intramuscular dose, raising a 1:853 mean titer of plaque reduction neutralization test. Moreover, outbred mice infected with one of three pathogenic reassortant ZH501 strains, which encoded rMP12-GM50 L-, M-, or S-segments, showed 90%, 50%, or 30% survival, respectively. These results indicate that attenuation of the rMP12-GM50 strain is significantly attenuated via the L-, M-, and S-segments. Recombinant RVFV vaccine strains encoding similar silent mutations will be also useful for the surveillance of reassortant strains derived from vaccine strains in endemic countries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 35, Issue 48, Part B, 4 December 2017, Pages 6634-6642
Journal: Vaccine - Volume 35, Issue 48, Part B, 4 December 2017, Pages 6634-6642
نویسندگان
Hoai J. Ly, Shoko Nishiyama, Nandadeva Lokugamage, Jennifer K. Smith, Lihong Zhang, David Perez, Terry L. Juelich, Alexander N. Freiberg, Tetsuro Ikegami,