The efficacy of HSV-2 vaccines based on gD and gB is enhanced by the addition of ICP27

► Vaccination with HSV gD, gB and ICP27 provided protection from HSV-2 challenge. ► Vaccination with VP13/14 or VP22 did not provide protection. ► Combining ICP27 with gD or gB provided a greater level of protection than any single antigen. ► Adding ICP27 to enhance T cell responses can improve the efficacy of gB- or gD-based HSV vaccines.