کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8490929 | 1552345 | 2018 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A small scale in vitro system for high throughput gas production analysis - A comparison with the Hohenheim gas test
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کلمات کلیدی
NIRSmaize gluten mealGS1MS1Hohenheim gas testGS2Feed digestibilityaNDFomMS2SBMHGTMGSADFomMGMSBPAutomation - اتوماسیونstandard deviation - انحراف معیارConcentrate - تمرکزGas production - تولید گازSugar beet pulp - خمیر چغندر قندRSM - روششناسی سطح پاسخWheat bran - سبوس گندمRumen - شکمبه، سیرابیCoefficient of Variation - ضریب تغییرScreening - غربالگریdry matter - ماده خشکApes - مردابLignocellulose - مواد لیگنوسلولزیNear Infrared spectroscopy - نزدیک به طیف سنجی مادون قرمزRapeseed meal - وعده غذایی کلزاSoybean meal - کنجاله سویاLucerne hay - یونجه لوسرنGrass hay - یونجه چمن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
An automated small scale in vitro system has been developed for the extensive examination of feedstuffs in high throughput screenings. This system was calibrated against the strictly standardized Hohenheim gas test (HGT). The automated system is suitable for gas-producing biological suspensions, e.g. ruminal fluid. Gas production is indirectly measured through pressure increase as a measure for digestibility. For functional characterisation of the micro gas system (MGS), a total number of 18 feedstuffs, including grass hay and a concentrate standard, were simultaneously incubated in the HGT and the MGS. The same inoculate and feed-inoculate ratio was applied for comparisons of kinetic parameters. Compared to HGT, the MGS resulted in significantly lower (pâ¯<â¯0.05) maximum gas production for all feedstuffs. Regarding the rate of gas production, the lag phase using the MGS was longer compared to HGT for 12 feeds. However, a strong relationship between the HGT and MGS gas production after 24â¯h was observed. The regression analysis of feedstuff mean values of both methods resulted in the equation: yâ¯=â¯0.91 (SEâ¯=â¯0.0342; pâ¯<â¯0.0001) x -0.03 (SEâ¯=â¯1.3924; pâ¯=â¯0.9823) with R²â¯=â¯0.98. The results indicate that the MGS is a suitable alternative method for high throughput screenings but also, with reservations, for questions which were formerly examined with the HGT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Feed Science and Technology - Volume 241, July 2018, Pages 8-14
Journal: Animal Feed Science and Technology - Volume 241, July 2018, Pages 8-14
نویسندگان
Karola Elberg, Patrick Steuer, Ute Habermann, Jürgen Lenz, Michael Nelles, Karl-Heinz Südekum,