کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8492983 | 1552792 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation, identification and pathogenicity characterization of Vibrio ponticus from the golden pompano Trachinotus ovatus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To determine the pathogeny for the mass death in the golden pompano Trachinotus ovatus in Hainan, China, the potential pathogens (HAINUV01, HAINUV02, and HAINUV03) were isolated from the infected pompano. The infected fish showed swollen, hyperemic livers and spleens, yellowish effusion in the gut, and different degrees of ulcerations on the bases of dorsal and pelvic fins. HAINUV01 had the strongest virulence to the pompano with a lethal dose (LD50) of 14â¯days of 1.75â¯Ãâ¯102â¯CFU/g fish by intraperitoneal (i.p.) injection. HAINUV01 was Gram-negative, straight rods, 2.37â¯Â±â¯0.12â¯Î¼m in length by 0.857â¯Â±â¯0.003â¯Î¼m width, with only one polar flagellum (4.78â¯Â±â¯0.33â¯Î¼m). Neighbor-joining phylogenetic analysis based on 16S rRNA, mreB, pyrH, recA and rpoA genes identified HAINUV01 as Vibrio ponticus. These findings provide direction for controlling V. ponticus-related disease occurrences in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 496, 1 November 2018, Pages 285-290
Journal: Aquaculture - Volume 496, 1 November 2018, Pages 285-290
نویسندگان
Shubin Liu, Erchao Li, Yan Cai, Shifeng Wang, Zhuling Ren, Qiming Li, Weiliang Guo, Yue Wu, Yongcan Zhou,