کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8493476 1552802 2018 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Disaccharide supplementation of extenders is an effective means of improving the cryopreservation of semen in sturgeon
ترجمه فارسی عنوان
مکمل دیساچاری افزودنیها یک وسیله موثر برای بهبود فریزر شدن مایع منی در ماهیان خاویاری است
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
چکیده انگلیسی
Sperm damage can be alleviated by optimizing the types and levels of cryoprotectants (including sugars) added to the extender to protect the sperm cells during freezing. This study was conducted to elucidate the effect of different disaccharides (maltose, lactose, trehalose and lactulose) upon the motility parameters of cryopreserved semen and the post-thaw storage of semen from Persian sturgeon, Acipenser persicus and Beluga sturgeon, Huso huso. Semen samples were diluted 1:5 in 9% methanol containing 0.2 M maltose, trehalose, lactose or lactulose. Then, semen was equilibrated for 10 min on ice and submerged in liquid nitrogen. After thawing (40 °C, 5 s) semen was stored for 0, 15, 30 and 60 min at 4 °C and semen motility investigated. For both sturgeon, the type of disaccharide had no effect on the duration of semen motility (expressed as curvilinear velocity, VCL). From the four disaccharides tested, lactulose exhibited the lowest sperm percentage. Furthermore, for all four disaccharides, cryopreserved semen motility was not affected by storage times of up to 30 min maximum; however, after 60 min of storage, a significant reduction was observed. Consequently, the best results were achieved with the disaccharides featuring glucose in their composition. In other words, maltose, lactose and trehalose can be used successfully for the cryopreservation of sturgeon semen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 486, 3 February 2018, Pages 261-265
نویسندگان
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