Validation of high throughput methods for tissue disruption and nucleic acid extraction for ranaviruses (family Iridoviridae)

► Semi-automated bead beating homogenisation technique is compatible with ranavirus detection. ► The most efficient method for tissue homogenisation and nucleic acid extraction for ranaviruses was semi-automated methods. ► Employment of bead beating and magnetic particle processing was the most economical and facilitated high through put.