کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8496764 | 1552984 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ranavirus detection by PCR in cultured tadpoles (Rana catesbeiana Shaw, 1802) from South America
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Diseases in farmed tadpoles (Rana catesbeiana) are a common event, being an economically important threat for Uruguayan and Brazilian farms. Based on clinical signs and epizootiology, pathogens belonging to the Family Iridoviridae were suspected as the possible etiology. Although these viruses have already been widely incriminated affecting aquatic organisms including frogs, their presence in Brazil and Uruguay was never mentioned so far. The objective of this work was to detect the presence of ranaviral agents in affected tadpoles using Polymerase Chain Reaction (PCR) technique as a primary approach to the study of the disease. Primers were designed based on highly conserved iridoviral sequences. Major Capsid Protein (MCP) and Immediate Early Protein (IE) genes were the selected targets. A positive PCR result was obtained for both genes when sick tadpoles from Brazil and Uruguay were analyzed. To confirm the amplification of an Iridoviridae, PCR products were purified and sequenced. Amplified products showed high degree of homology with several members of the Iridoviridae, mostly with those belonging to the genus Ranavirus. Obtained sequences were registered in the GenBank with accession nos. AY585203, AY585204 and AY744387. This report indicates that Ranavirus should be considered into the aquatic organism disease etiologies throughout this geographical region.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 257, Issues 1â4, 30 June 2006, Pages 78-82
Journal: Aquaculture - Volume 257, Issues 1â4, 30 June 2006, Pages 78-82
نویسندگان
Leonardo Galli, Alfredo Pereira, Alejandro Márquez, Rolando Mazzoni,