کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8499551 | 1553627 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis)
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کلمات کلیدی
HcyFCMstandard deviation - انحراف معیارTem - این استEnzymatic assay - تست آنزیمیNeutral red - خنثی قرمزPhagocytosis - فاگوسیتوز یا بیگانهخواری Flow cytometry - فلوسیتومتریphenoloxidase - فنلوکسیدازSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیProtease inhibitor - مهارکننده پروتئازScanning electron microscopy - میکروسکوپ الکترونی روبشیTransmission electron microscopy - میکروسکوپ الکترونی عبوریproPO - نزدیکMPs - نمایندگان مجلسHemocyanin - هموسیانینHemocyte - هموسیتprophenoloxidase - پروپنولوکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis) Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis)](/preview/png/8499551.png)
چکیده انگلیسی
Little is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 40, Issue 2, October 2014, Pages 362-373
Journal: Fish & Shellfish Immunology - Volume 40, Issue 2, October 2014, Pages 362-373
نویسندگان
Charles Le Pabic, Didier Goux, Maryline Guillamin, Georges Safi, Jean-Marc Lebel, Noussithé Koueta, Antoine Serpentini,