Establishment of a robotic, high-throughput platform for the specific diagnosis of gastrointestinal nematode infections in sheep

► A robotic, high throughput molecular assay for diagnosis of infections with ovine gastrointestinal nematodes is assessed. ► This assay is used to investigate the epidemiology of gastrointestinal parasites of sheep in Australia. ► The molecular results are directly compared with those of routine larval culture. ► The results achieved by larval culture are influenced by the developmental success of different species. ► This molecular diagnostic platform represents a rapid, user-friendly diagnostic tool for routine diagnosis and epidemiology.