کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8500307 | 1553734 | 2008 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei](/preview/png/8500307.png)
چکیده انگلیسی
The mitochondrial RNA-binding proteins (MRP) 1 and 2 play a regulatory role in RNA editing and putative role(s) in RNA processing in Trypanosoma brucei. Here, we report the purification of a high molecular weight protein complex consisting solely of the MRP1 and MRP2 proteins from the mitochondrion of T. brucei. The MRP1/MRP2 complex natively purified from T. brucei and the one reconstituted in Escherichia coli in vivo bind guide (g) RNAs and pre-mRNAs with dissociation constants in the nanomolar range, and efficiently promote annealing of pre-mRNAs with their cognate gRNAs. In addition, the MRP1/MRP2 complex stimulates annealing between two non-cognate RNA molecules suggesting that along with the cognate duplexes, spuriously mismatched RNA hybrids may be formed at some rate in vivo. A mechanism of catalysed annealing of gRNA/pre-mRNA by the MRP1/MRP2 complex is proposed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 38, Issues 8â9, July 2008, Pages 901-912
Journal: International Journal for Parasitology - Volume 38, Issues 8â9, July 2008, Pages 901-912
نویسندگان
Alena ZÃková, Jana KopeÄná, Maria A. Schumacher, Kenneth Stuart, LukáÅ¡ TrantÃrek, Julius LukeÅ¡,