کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8505619 | 1555309 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of a peroxygen-based disinfectant for inactivation of porcine epidemic diarrhea virus at low temperatures on metal surfaces
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Porcine epidemic diarrhea virus (PEDV) spread rapidly across the United States in part due to contaminated livestock trailers. The objective of this study was to test a peroxygen-based disinfectant for the ability to inactivate PEDV on aluminum surfaces at 4â¯Â°C or â10â¯Â°C. Forty 3-week-old individually housed barrows were used as a bioassay to determine the infectivity of PEDV after treatment with either a 1:100 or 1:600 dilution of a peroxygen-based disinfectant with 10 or 30â¯min of contact time. One coupon matched to one pig was the experimental unit. Coupons in the positive control and disinfectant treatment groups were contaminated with 2â¯mL of feces spiked with PEDV. A negative control group was contaminated with PEDV-negative feces. Following treatment, the feces and disinfectant remaining in the coupons was collected and administered to pigs intragastrically. Rectal swabs were collected from pigs 3 and 7â¯days post-inoculation (DPI) and tested for PEDV by RT-qPCR. Samples from all coupons, except the negative control, were positive by RT-qPCR for PEDV before and after treatment. All rectal swabs from the pigs in the negative control and the seven disinfectant treatment groups were RT-qPCR negative for PEDV on 3 and 7 DPI. All pigs in the positive control at 4â¯Â°C and 3 of 4 pigs in the positive control conducted at â10â¯Â°C were RT-qPCR positive for PEDV on 3 and 7 DPI. Both the 1:100 and 1:600 dilutions of peroxygen-based disinfectant successfully inactivated PEDV under the conditions of this study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 214, February 2018, Pages 99-107
Journal: Veterinary Microbiology - Volume 214, February 2018, Pages 99-107
نویسندگان
Kimberlee L. Baker, Christine L. Mowrer, Jianqiang Zhang, Qi Chen, Alejandro Ramirez, Chong Wang, Locke A. Karriker, Derald J. Holtkamp,