کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8505850 1555394 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Haemophilus parasuis exhibits IgA protease activity but lacks homologs of the IgA protease genes of Haemophilus influenzae
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Haemophilus parasuis exhibits IgA protease activity but lacks homologs of the IgA protease genes of Haemophilus influenzae
چکیده انگلیسی
Haemophilus parasuis, the bacterium responsible for Glässer's disease, is a pathogen of significant concern in modern high-health swine production systems but there is little information regarding the identity or function of its virulence factors. Several important human mucosal pathogens, including the closely related bacterium Haemophilus influenzae, utilize IgA proteases to aid in defeating the host immune response and facilitate disease but it is unknown whether H. parasuis synthesizes any product with IgA protease activity. To investigate potential virulence mechanisms of H. parasuis, we evaluated five strains for their ability to digest purified IgA. Western blotting demonstrated cleavage of swine IgA, but not human IgA1, following incubation with culture supernatants from three strains, two of which are known to cause invasive disease. No genes with homology to the H. influenzae IgA protease genes iga and igaB could be identified in any H. parasuis strain using either PCR or Southern blotting. These results demonstrate that a novel IgA protease produced by some strains of H. parasuis cleaves the swine IgA heavy chain at a site not found in human IgA1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 153, Issues 3–4, 15 December 2011, Pages 407-412
نویسندگان
, , , ,