کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8521123 1557719 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
پیش نمایش صفحه اول مقاله
Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry
چکیده انگلیسی
Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Analysis - Volume 8, Issue 1, February 2018, Pages 69-74
نویسندگان
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