کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8526072 | 1557941 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Halofuginone attenuates intervertebral discs degeneration by suppressing collagen I production and inactivating TGFβ and NF-кB pathway
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کلمات کلیدی
CEPSNF-κBIL-6TGFβIVDIL-1βHalofuginoneECMIL-8NF-кB - NF-kBImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیinterleukin 1β - اینترلوکین 1βinterleukin 6 - اینترلوکین 6Interleukin 8 - اینترلوکین 8dHI - بزtumor necrosis factor α - تومور نکروز عامل αIntervertebral discs - دیسک های بین مهره ایTNF-α - فاکتور نکروز توموری آلفاAnnulus fibrosus - فیبروروز انولوسExtracellular matrix - ماتریکس خارج سلولیCollagen I - کلاژن من
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Most low back pain is caused by intervertebral discs (IVD) degeneration, a disease that prevalence is increasing with age. Halofuginone, an analog of ferbrifugine isolated from plant Dichroa febrifuga, has drawn much attention in recent years for the wide range of bioactivities in malaria, cancer, fibrotic and autoimmune diseases. In this study, we evaluated the benefit effects of halofuginone in IVD degeneration treatment in a validated rabbit puncture model. Halofuginone treatment could attenuate disc degeneration by suppressing the decrease of discs height and nucleus pulposus signal strength. Besides, halofuginone treatment could suppress mRNA and protein expression of collagen I in nucleus pulposus. This might possibly due to the inactivation of transform growth factor-β (TGFβ) signal pathway by down-regulating p-Samd3 and up-regulating inhibitory Smad7. Then, we evaluated the effects of halofuginone treatment on nuclear factor of kappa B (NF-κB) signal pathway and its downstream pro-inflammatory cytokines. The level of p-p65 and p-IκBα was down-regulated in halofuginone treated group, indicating the inactivation of NF-κB signal pathway. The mRNA expression of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and interleukin 8 (IL-8) was decreased in nucleus pulposus too, indicating the down-regulation of pro-inflammatory cytokines. In conclusion, halofuginone treatment could attenuate IVD degeneration and this was possibly due to suppressing of collagen I production and inactivation of TGFβ and NF-κB signal pathway in nucleus pulposus of degenerated discs. These results suggest that halofuginone has the potential for IVD degeneration treatment, but more research is needed to validate this.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 101, May 2018, Pages 745-753
Journal: Biomedicine & Pharmacotherapy - Volume 101, May 2018, Pages 745-753
نویسندگان
Linghui Luo, Yong Gao, Cao Yang, Zengwu Shao, Xinghuo Wu, Shuai Li, Liming Xiong, Chao Chen,