کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8531205 | 1559731 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tn (N-acetyl-d-galactosamine-O-serine/threonine) immunization protects against hyperoxia-induced lung injury in adult mice through inhibition of the nuclear factor kappa B activity
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کلمات کلیدی
PCNAIκB-αPBSMUCHRPNF-κBProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیimmunoglobulin - ایمونوگلوبولینMLI - این موضوعinterleukin - اینترلوکینBALF - بافتEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاNuclear factor-kappa B - فاکتور هسته ای-کاپا BBronchoalveolar lavage fluid - مایع لارو برونکلوفلورmean linear intercept - متوسط رهگیری خطیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMucin - موسین room air - هوا اتاقHorseradish peroxidase - پراکسیداز هوررادیش
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Prolonged hyperoxia exposure leads to inflammation and acute lung injury. Since hyperoxia activates nuclear factor kappa B (NF-κB) and proinflammatory mediators in lung fibroblasts and murine lungs, and proinflammatory cytokines upregulate Tn (N-acetyl-d-galactosamine-O-serine/threonine) expression in human gingival fibroblasts. We hypothesized connections exist between Tn expression and inflammation regulation. Thus, we immunized adult mice with Tn antigen to examine whether Tn vaccine can protect against hyperoxia-induced lung injury by inhibiting NF-κB activity and cytokine expression through the action of anti-Tn antibodies. Five-week-old female C57BL/6NCrlBltw mice were subcutaneously immunized with Tn antigen four times at biweekly intervals, and one additional immunization was performed at 1â¯week after the fourth immunization. Four days after the last immunization, mice were exposed to room air (RA) or hyperoxia (100% O2) for up to 96â¯h. Four study groups were examined: carrier proteinâ¯+â¯RA (nâ¯=â¯6), Tn vaccineâ¯+â¯RA (nâ¯=â¯6), carrier proteinâ¯+â¯O2 (nâ¯=â¯6), and Tn vaccineâ¯+â¯O2 (nâ¯=â¯5). We observed that hyperoxia exposure reduced body weight, increased alveolar protein and cytokine (interleukin-6 and tumor necrosis factor-α) levels, increased mean linear intercept (MLI) values and lung injury scores, and increased lung NF-κB activity. By contrast, Tn immunization increased serum anti-Tn antibody titers and reduced the cytokine levels, MLI values, and lung injury scores. Furthermore, the alleviation of lung injury was accompanied by a reduction in NF-κB activity. Therefore, we proposed that Tn immunization attenuates hyperoxia-induced lung injury in adult mice by inhibiting the NF-κB activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 59, June 2018, Pages 261-268
Journal: International Immunopharmacology - Volume 59, June 2018, Pages 261-268
نویسندگان
Chung-Ming Chen, Jaulang Hwang, Hsiu-Chu Chou, Her-Shyong Shiah,