کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8553811 | 1562695 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of surface charge and pH on the physiological behaviour of cobalt, copper, manganese, antimony, zinc and titanium oxide nanoparticles in vitro
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کلمات کلیدی
DLSBrunauer–Emmett–Teller methodMeOxNEAAIEPMTSFBSSDS3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium - 3- (4،5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H-tetrazoliumBSA - BSACCm - CCMAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPPest - آفاتbovine serum albumin - آلبومین سرم گاوELS - آنهاnon-essential amino acids - اسید آمینه غیر ضروریHydrodynamic size - اندازه هیدرودینامیکیMetal oxide - اکسید فلزsodium dodecyl sulphate - سدیم دودسیل سولفاتfetal bovine serum - سرم جنین گاوSurface charge - سطح شارژBET - شرطlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH complete culture medium - محیط کشت کاملNanoparticle - نانوذارتIsoelectric point - نقطه ایزوالکتریکElectrophoretic light scattering - پراکندگی نور الکتروفورزDynamic Light Scattering - پراکندگی نور دینامیکی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The precise knowledge on various interactions of metal nanoparticles (NP) in a living organism is scarce. It is expected that metals can bind to nucleic acids, peptides and proteins (e.g. enzymes), and modify the functioning of vital cellular compartments after entering the organism. The predictive factors for quantitative nanostructure-activity relationship (QNAR) analysis could enhance efficient and harmless usage of nanoparticles (NPs) in the industry as well in the medicine. The studies value the composition of the NP corona determined by time, temperature and source of protein which has been found to implicate the physiological behaviour of NPs. One has largely been ignored: the NPs specific isoelectric point (IEP) and pH at the state of measurement. Herein, this study investigates the effect of pH and surface charge of six metal oxide (MeOx) NPs on time dependency of cytotoxicity. Several aspects of the characterization of ultrafine particles in the actual test system which is the most relevant for the interpretation of the toxicological data are referred: (i) the difference of pH in the room temperature and in the incubation conditions (ii) the difference of dispersions in MilliQ and complete cell media; (iii) the need to exemplify also the pH and isoelectric point when the hydrodynamic size is measured; (iv) the importance of time due to the time-dependent equilibration and changes of NPs corona. The surface charge determines the formation of corona and could be modified by pH. MeOx NPs without fully charge equilibrated corona might play the main role of MeOx NPs entering into the cell and consequently the time dependent manifestation of the cellular effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 50, August 2018, Pages 11-21
Journal: Toxicology in Vitro - Volume 50, August 2018, Pages 11-21
نویسندگان
Tiina Titma,