کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8554136 | 1562698 | 2018 | 43 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of candidate reference chemicals for in vitro steroidogenesis assays
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کلمات کلیدی
OECDU.S. EPAH295REDSP17β-estradiol - 17β استرادیولandrogens - آندروژنUnited States Environmental Protection Agency - آژانس حفاظت از محیط زیست ایالات متحدهSteroidogenesis - استروئیدوژنزEstrone - استرونEstrogens - استروژنandrostenedione - اندرسوندیونEndocrine Disruptor Screening Program - برنامه غربالگری غشای مزمنtestosterone - تستوسترونOrganization for Economic Cooperation and Development - سازمان همکاری های اقتصادی و توسعهSex hormones - هورمونهای جنسی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The Endocrine Disruptor Screening Program (EDSP) is transitioning from traditional testing methods to integrating ToxCast/Tox21 in vitro high-throughput screening assays for identifying chemicals with endocrine bioactivity. The ToxCast high-throughput H295R steroidogenesis assay may potentially replace the low-throughput assays currently used in the EDSP Tier 1 battery to detect chemicals that alter the synthesis of androgens and estrogens. Herein, we describe an approach for identifying in vitro candidate reference chemicals that affect the production of androgens and estrogens in models of steroidogenesis. Candidate reference chemicals were identified from a review of H295R and gonad-derived in vitro assays used in methods validation and published in the scientific literature. A total of 29 chemicals affecting androgen and estrogen levels satisfied all criteria for positive reference chemicals, while an additional set of 21 and 15 chemicals partially fulfilled criteria for positive reference chemicals for androgens and estrogens, respectively. The identified chemicals included pesticides, pharmaceuticals, industrial and naturally-occurring chemicals with the capability to increase or decrease the levels of the sex hormones in vitro. Additionally, 14 and 15 compounds were identified as potential negative reference chemicals for effects on androgens and estrogens, respectively. These candidate reference chemicals will be informative for performance-based validation of in vitro steroidogenesis models.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 47, March 2018, Pages 103-119
Journal: Toxicology in Vitro - Volume 47, March 2018, Pages 103-119
نویسندگان
Caroline Lucia Pinto, Kristan Markey, David Dix, Patience Browne,