کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8625216 | 1568112 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Osteopontin regulates dentin and alveolar bone development and mineralization
ترجمه فارسی عنوان
استئپونتین دندان ها و استخوان های آلوئولی را تنظیم و کانی سازی می کند
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کلمات کلیدی
LCMCOL1A1PDLSLRPENPP1OPNECMANKBSPISHTNAPPPIEctonucleotide pyrophosphatase phosphodiesterase 1Collagen XIISmall Integrin-Binding LIgand N-linked GlycoproteinDCNfmodH&E - H & EIn situ hybridization - Hybridization در محلLC-MS/MS - LC-MS / MSpicrosirius red - picrosirius قرمزTissue-nonspecific alkaline phosphatase - آلکالین فسفاتاز غیر اختصاصی بافتیOsteopontin - استئوپنتینIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیCEJ - بیشترTooth development - توسعه دندانToluidine blue - تولویدین آبیMicro-computed tomography - توموگرافی کامپیوتری میکروSibling - خواهر و برادرdecorin - دکورینPeriodontal ligament - رباط پریودنتالBone biology - زیست شناسی استخوانbone sialoprotein - سیلوپروتئین استخوانdental cementum - سیمان دندانLiquid chromatography-tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع دو طرفهFibromodulin - فیبر مولدولینlaser capture microdissection - لیزر ضبط میکرو دیسکسیونExtracellular matrix - ماتریکس خارج سلولیMineralization - معدنی سازی یا کانی سازیMicro-CT - میکرو CTwild-type - نوع وحشیCollagen type 1 - نوع کلاژن 1Hematoxylin and Eosin - هماتوکسیلین و ائوزینsmall leucine-rich proteoglycan - پروتئگلیکان غنی از لوسین استPeriostin - پریستینPostn - پست پستinorganic pyrophosphate - پیرو فسفات معدنی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
چکیده انگلیسی
The periodontal complex is essential for tooth attachment and function and includes the mineralized tissues, cementum and alveolar bone, separated by the unmineralized periodontal ligament (PDL). To gain insights into factors regulating cementum-PDL and bone-PDL borders and protecting against ectopic calcification within the PDL, we employed a proteomic approach to analyze PDL tissue from progressive ankylosis knock-out (Ankâ/â) mice, featuring reduced PPi, rapid cementogenesis, and excessive acellular cementum. Using this approach, we identified the matrix protein osteopontin (Spp1/OPN) as an elevated factor of interest in Ankâ/â mouse molar PDL. We studied the role of OPN in dental and periodontal development and function. During tooth development in wild-type (WT) mice, Spp1 mRNA was transiently expressed by cementoblasts and strongly by alveolar bone osteoblasts. Developmental analysis from 14 to 240Â days postnatal (dpn) indicated normal histological structures in Spp1â/â comparable to WT control mice. Microcomputed tomography (micro-CT) analysis at 30 and 90Â dpn revealed significantly increased volumes and tissue mineral densities of Spp1â/â mouse dentin and alveolar bone, while pulp and PDL volumes were decreased and tissue densities were increased. However, acellular cementum growth was unaltered in Spp1â/â mice. Quantitative PCR of periodontal-derived mRNA failed to identify potential local compensators influencing cementum in Spp1â/â vs. WT mice at 26Â dpn. We genetically deleted Spp1 on the Ankâ/â mouse background to determine whether increased Spp1/OPN was regulating periodontal tissues when the PDL space is challenged by hypercementosis in Ankâ/â mice. Ankâ/â; Spp1â/â double deficient mice did not exhibit greater hypercementosis than that in Ankâ/â mice. Based on these data, we conclude that OPN has a non-redundant role regulating formation and mineralization of dentin and bone, influences tissue properties of PDL and pulp, but does not control acellular cementum apposition. These findings may inform therapies targeted at controlling soft tissue calcification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 107, February 2018, Pages 196-207
Journal: Bone - Volume 107, February 2018, Pages 196-207
نویسندگان
B.L. Foster, M. Ao, C.R. Salmon, M.B. Chavez, T.N. Kolli, A.B. Tran, E.Y. Chu, K.R. Kantovitz, M. Yadav, S. Narisawa, J.L. Millán, F.H. Jr, M.J. Somerman,