کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8631496 | 1568853 | 2017 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Time- and dose-related effects of a gonadotropin-releasing hormone agonist and dopamine antagonist on reproduction in the Northern leopard frog (Lithobates pipiens)
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کلمات کلیدی
LHbIUCNBWTRPL8Ribosomal protein L8GnRH-aTMSDEPCfshbNCBIDRD2mRNANaClSVLqPCRGnRHInternational Union for the Conservation of NaturecDNA - cDNAComplementary DNA - DNA تکمیلیGonadotropin-releasing hormone agonist - آگونیست هورمون آزاد کننده گنادوتروپینBasic Local Alignment Search Tool - ابزار جستجوی محلی سازگاری محلیRNA - اسید ریبونوکلئیکribonucleic acid - اسید ریبونوکلئیکBlast - انفجارanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceintraperitoneal - داخل صفاقیDopamine - دوپامینdiethylpyrocarbonate - دیاتیلپیر کربناتmessenger ribonucleic acid - رسوب ریبونوکلئیک اسیدSaline - سالینsnout-vent length - طول خروجی دریچهMetoclopramide - متکللوپرامیدNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیMET - ملاقات کردSpawning - نشتGonadotropin-releasing hormone - هورمون آزاد کننده گنادوتروپینfollicle-stimulating hormone - هورمون تحریک کننده فولیکولluteinizing hormone - هورمون جسم زردFSH - هورمون محرکه فولیکولی quantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیbody weight - وزن بدنCaptive breeding - پرورش انفرادیdopamine receptor D2 - گیرنده Dopamine D2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Gonadotropin-releasing hormone (GnRH) stimulates luteinizing hormone release to control ovulation and spermiation in vertebrates. Dopamine (DA) has a clear inhibitory role in the control of reproduction in numerous teleosts, and emerging evidence suggests that similar mechanisms may exist in amphibians. The interactions between GnRH and DA on spawning success and pituitary gene expression in the Northern leopard frog (Lithobates pipiens) were therefore investigated. Frogs were injected during the natural breeding season with a GnRH agonist [GnRH-A; (Des-Gly10, D-Ala6, Pro-NHEt9)-LHRH; 0.1 μg/g and 0.4 μg/g] alone and in combination with the dopamine receptor D2 antagonist metoclopramide (MET; 5 μg/g and 10 μg/g). Injected animals were allowed to breed in outdoor mesocosms. Time to amplexus and oviposition were assessed, and egg mass release, incidences of amplexus, egg mass weight, total egg numbers and fertilization rates were measured. To examine gene expression, female pituitaries were sampled at 12, 24 and 36 h following injection of GnRH-A (0.4 μg/g) alone and in combination with MET (10 μg/g). The mRNA levels of the genes lhb, fshb, gpha, drd2 and gnrhr1 were measured using quantitative real-time PCR. Data were analyzed by a two-way ANOVA. Both GnRH-A doses increased amplexus, oviposition and fertilization alone. Co-injection of MET with GnRH-A did not further enhance spawning success. Injection of GnRH-A alone time-dependently increased expression of lhb, fshb, gpha and gnrhr1. The major effect of MET alone was to decrease expression of drd2. Importantly, the stimulatory effects of GnRH-A on lhb, gpha and gnrhr1 were potentiated by the co-injection of MET at 36 h. At this time, expression of fshb was increased only in animals injected with both GnRH-A and MET. Spawning success was primarily driven by the actions of GnRH-A. The hypothesized inhibitory action of DA was supported by pituitary gene expression analysis. The results from this study provide a fundamental framework for future time- and dose-response investigations to improve current spawning methods in amphibians.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 254, 1 December 2017, Pages 86-96
Journal: General and Comparative Endocrinology - Volume 254, 1 December 2017, Pages 86-96
نویسندگان
Maria Vu, Bradley Weiler, Vance L. Trudeau,