کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644567 | 1569763 | 2018 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genome-wide analysis of maize CONSTANS-LIKE gene family and expression profiling under light/dark and abscisic acid treatment
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کلمات کلیدی
ABAqRT-PCRWGDamino acid - آمینو اسیدabscisic acid - اسید آبسزیکGenome-wide analysis - تجزیه و تحلیل گسترده ژنومcoding sequence - توالی کدگذاریwhole genome duplication - تکثیر ژنوم کلMaize - ذرت یا جواریquantitative reverse transcription-PCR - رونویسی معکوس PCRTranscription factor - عامل رونویسیSynonymous - مترادفLight regulation - مقررات نورmya - میاmillion years ago - میلیون سال پیشnonsynonymous - نامشخصIsoelectric point - نقطه ایزوالکتریکNeighbor joining - پیوستن به همسایهCdS - کادمیم سولفید، سولفید کادمیمcol - کول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The CONSTANS-LIKE (COL) gene has an important role both in regulation flowering through photoperiodic control and response to abiotic stress. In the present study, we performed a genome-wide analysis of maize COL gene family and identified 19 non-redundant ZmCOL genes, which were unequally distributed on ten maize chromosomes. Analysis of compound phylogenetic tree (maize, sorghum, rice and Arabidopsis) showed high bootstrap, as well as conserved domain and semblable gene structures among members within the same clade, revealing that COL genes in same clade were from the common ancestral and prior to the divergence of monocots and dicots lineages. Calculation of Ka/Ks ratio demonstrated that most duplicated ZmCOLs experienced purifying selection, which suggested limited functional divergence after duplication events. Comparing interspecies gene collinearity between three major grasses species, extensive microsynteny was detected among maize, sorghum and rice COL-containing segments. Additionally, several light-responsive and one ABA-responsive cis-elements could be detected for ZmCOL genes in group A. Therefore, qRT-PCR was performed to explore the expression patterns of ZmCOL genes in group A under light/dark conditions and ABA treatment. Our results laid the foundation for functional characterization of ZmCOL proteins and uncovering the biological roles of COL genes in response to stress in future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 673, 5 October 2018, Pages 1-11
Journal: Gene - Volume 673, 5 October 2018, Pages 1-11
نویسندگان
Nannan Song, Zhilan Xu, Jing Wang, Qianqian Qin, Haiyang Jiang, Weina Si, Xiaoyu Li,