کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644863 | 1569770 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds
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کلمات کلیدی
tRNAACCaseqRT-PCRALAPLCsnRNAsnoRNAPHSncRNASPLLPAATSquamosa Promoter Binding Protein-LIKEPDHcPaeoniarRNAβ-ketoacyl-ACP synthaseβ-ketoacyl-ACP reductaselncRNACCTACPEnoyl-ACP reductase - Enoyl-ACP ردوکتازRibosomal RNA - RNA RibosomalNon-coding RNA - RNA غیرکد کننده، RNA غیرترجمه شوندهsmall nuclear RNA - RNA هسته ای کوچکsmall nucleolar RNA - RNA کوچک هسته ایacetyl-CoA carboxylase - استیل کروکسی سیلازα-linolenic acid - اسید α-لینولنیکOleic acid - اسید اولئیکLinoleic acid - اسید لینولئیکFatty acid - اسید چربtransfer RNA - انتقال RNAtriacylglycerol - تری آسیل گلیسرول TAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولHAD - داشته استKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوphospholipase C - فسفولیپاز Clysophosphatidic acid acyltransferase - لیسفسفید فسفیدید اسید آتیل ترانسفرازMiRNA - میکروRNA، ریزآرانای، miRNAGene ontology - هستیشناسی ژنیquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیacyl carrier protein - پروتئین حامل آکیلpyruvate dehydrogenase - پیرووات دهیدروژنازKAS - چیTarget gene - ژن هدفKar - کارEAR - گوش
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) act as important molecular regulators in a wide range of biological processes during plant development and seed formation, including oil production. Tree peony seeds contain >90% unsaturated fatty acids (UFAs) and high proportions of α-linolenic acid (ALA, > 40%). To dissect the non-coding RNAs (ncRNAs) pathway involved in fatty acids synthesis in tree peony seeds, we construct six small RNA libraries and six transcriptome libraries from developing seeds of two cultivars (J and S) containing different content of fatty acid compositions. After deep sequencing the RNA libraries, the ncRNA expression profiles of tree peony seeds in two cultivars were systematically and comparatively analyzed. A total of 318 known and 153 new miRNAs and 22,430 lncRNAs were identified, among which 106 conserved and 9 novel miRNAs and 2785 lncRNAs were differentially expressed between the two cultivars. In addition, potential target genes of the microRNA and lncRNAs were also predicted and annotated. Among them, 9 miRNAs and 39 lncRNAs were predicted to target lipid related genes. Results showed that all of miR414, miR156b, miR2673b, miR7826, novel-m0027-5p, TR24651|c0_g1, TR24544|c0_g15, and TR27305|c0_g1 were up-regulated and expressed at a higher level in high-ALA cultivar J when compared to low-ALA cultivar S, suggesting that these ncRNAs and target genes are possibly involved in different fatty acid synthesis and lipid metabolism through post-transcriptional regulation. These results provide a better understanding of the roles of ncRNAs during fatty acid biosynthesis and metabolism in tree peony seeds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 666, 5 August 2018, Pages 72-82
Journal: Gene - Volume 666, 5 August 2018, Pages 72-82
نویسندگان
Dan-Dan Yin, Shan-Shan Li, Qing-Yan Shu, Zhao-Yu Gu, Qian Wu, Cheng-Yong Feng, Wen-Zhong Xu, Liang-Sheng Wang,